BtaINT0029293 @ bosTau6
Intron Retention
Gene
ENSBTAG00000012210 | C5
Description
complement C5a anaphylatoxin precursor [Source:RefSeq peptide;Acc:NP_001160088]
Coordinates
chr8:112296555-112298535:-
Coord C1 exon
chr8:112298338-112298535
Coord A exon
chr8:112296646-112298337
Coord C2 exon
chr8:112296555-112296645
Length
1692 bp
Sequences
Splice sites
5' ss Seq
TGCGTAAGT
5' ss Score
9.73
3' ss Seq
CATATATACTTTCCCCCCAGATA
3' ss Score
9.53
Exon sequences
Seq C1 exon
CTGCTAAATACAGAAACGCATGGGTGAAGAAGTGTTGTTATGATGGAGCCCATCGGAATGATGATGAAACCTGTGAGCAGCGAGCCGCTCGGATTCAGGCAGGCCCCATATGTATCAAAGCTTTCAAGAGCTGTTGTGCCATCGCAAGCCAGTTCCGGGCTGATGAGCATCACAAAAATATGCAACTAGGAAGGCTGC
Seq A exon
GTAAGTTTGGCATTTTCTATTGGAAAATATGGAAAATTTGATGTCACTTTATGCTGCCAAAAAAGGCAGTGTTCATGCTCTACTTAAAATGGAAAGTAGAAAAGATAACTTGTGAATGCTCTTAAGAAAAAAAATTGTTGTCACTTTGGGGAATCAGAAGAGGTGAATACAGAACAGAGCAGAGCGCTCTCGTCCGTAGTTAAGACCCAGATAGGTCTGAAGCTGACTCAGCCCATAAACGTGAATGGTTTTTCATCCTCTCTGAGCCTCACTCTCCTCATTTTTAAAGCAAGAAACTTGGGCCAGATGATATCTAATATGCCTTTGGGTTCTACAATTTCATGATTCTGTGATATTACAACCATTAAAATCCCGTTTGTACCTATACTGACCAAGTATAGCAGAAGATTTCAAAGTGTAGCATTAAGTCAAATGTACTTACGTACAAGTTGAAATCGTATTTCAATTGAAACCACCATATACATCTTCAGAATTAACAGGTTTTCAGAACTTTTAGGACTTTCAATTCATTCACATACAGTCAAAACAAACAAAAACGGAACGATAGACCCTGCTTGACTGTTTTGCTTAACAGTACGTCTTAAACGTTATCGTTCTGAGTGGGTTTATTTCTGCAGAAATAAACTCCTGTGGCCATTTGTTGAAACCAAGTCATTAGATAGATGTAAGCTGTTTTCAGACTTTTGCTTATTACGCGCAGTATTTTGTATGAACAACCTAGTGCATATCCCTTTCTCGAGGTCCAGGTCTTCAACTCTAACCATCTGGGTTGGCATCAGTTGCCTGGAATTCAGCCTCTCCAGTTCCCGCCTCTGTTCCTTGTTCTAGACAGCTTAGCCTCCCAAATCTCACGCCTGGTGTTGCAACTCCGCCGGGGTCTGTATGGGTTTCTCTCCCTGCTCTGAAGCCTGTAAAATGCCTCCAGATAGTAAACCTGGATAATTTTGGGGTTTACCTAATCTGTCTCCCTTCCCTGAGGGATCCCTGATCTGGCTCACTTAAGCTCTAATATCTGAAAGCAAGCTATTGTGTCTGGTTTTCTAGTTTTTTTTAAGGCAGAGAAGTAACTCTAGTCCCTATCAGAACATCTTGGACATAAGCAGATATTCTGCTCATTTTTATTAAAGGAAATATAAATAAATGTCTGCAGTAAGGCAAAGAATCACATAAACTTCCCTATTTTATAAAATAATAACAATTGTTGCAATTTTTAAATGTAAATAACTTTTTAAAATTTTACTTATTTATTTATTTGGCTGCACTGGGTCTTAGTTATGGCATGTGGGATCTAGTTCCCTGACCAGGGGTCAAACCCAGGTCCCCTGCAGCAGGAGCCCAGACTCATAGCCACTGGACCACCAGGAAATCCCTGCAATAGACAGGAACAGACTTTATAGACTAATTAGTATTTTGGACAATTTTCAGAACATGAAACATTTAGCAATTTAGAAAAAAATTTATTAAATATATTTTTATGCTTTATTCATTTTGTCAGTGCTGCTAGTGGAGAAATCCTAAGGCCTTTATTGTTCTATTTTGAAACTGCTAAACAAAATGTTTCACTGACCTTTCAGAAAATACTTAAGCTATTGATTAATGTCTACATTTAAAAATTCAGCCCTAGGTAGAATTTTTGCTGTTTTTCCATAATCATATATACTTTCCCCCCAG
Seq C2 exon
ATATAAAGTCTCTTTTACCAGTAACCAAACCAGAAATAAGAAGCTATTTTCCAGAAAGCTGGCTATGGGAAGTTCATCGTGTCCCGAAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000012210:ENSBTAT00000016204:17
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.030 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0182113=ANATO=WD(100=52.2)
A:
NA
C2:
PF0020717=A2M=PU(12.5=35.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCTAAATACAGAAACGCATGGGT
R:
GGGACACGATGAACTTCCCAT
Band lengths:
282-1974
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]