BtaINT0029300 @ bosTau6
Intron Retention
Gene
ENSBTAG00000012210 | C5
Description
complement C5a anaphylatoxin precursor [Source:RefSeq peptide;Acc:NP_001160088]
Coordinates
chr8:112277811-112279439:-
Coord C1 exon
chr8:112279350-112279439
Coord A exon
chr8:112278024-112279349
Coord C2 exon
chr8:112277811-112278023
Length
1326 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAAT
5' ss Score
8.88
3' ss Seq
TGTGTGCTTGTTTGTTTCAGGAC
3' ss Score
11.76
Exon sequences
Seq C1 exon
GCATCATTAGCAGGCGAAAGGAGTTCCCATACAGGATACCATTAAATATGGTCCCCAAAACAAAAGTCAAAAGAACTGTGAGTATAAAAG
Seq A exon
GTAAATTAACCAGTGCCATTTTTATTTCTTATTTCACTGTGTTTCAAACAAAAGTCAAGTATTCTCATGACTTTCCCATTTCTCCTCTTCTCTACTCAGTTGCATATCCAACGGGGGCTCTGGGAGTGCCCTGTTCCTGCCCTCACCTTCACTTCCCCAAGCTCACTTTCAGCTCTCTTCTTCCAGGGGCTTGATTTCATGGAGATTCATGCTCCCATGATGGAGGGGGGGGGGGGGGCTGTCACTGGCCCAAAGACTTCTCTAACATGAGAGTGCAGCTGCGTCCAGATTTAAAGGACTTTCTATGATCCAAGTGGGCAGTCTTAGTCCAGAAACAGTTGCATGCCCTGCAAATGGCCCCAGGAAAGCAGCTCTGGGGCCATCTGTAGCACTGGTGTTTTTAGTGGGCTTTATCCCTGGCCTCCAGCCTTTAAAAATTCAAGGACTTATCATCACAAGAATAGAATGTCCTGACTACATTTTATCTCAGATAAAAGCTTGGAATCACAGAATGTTAGTACTGGAAAGGACCTTTCTAAGCATGCCCCACCCTGTTGTTGGATATATAAGAGAGTGGTTTTCCCAGGTCTCACGATGAGATATAGACAGAGCCCTCATGGCATTCACAGAGCGTTCACAGTTCAGACTCTTGGCTTTGCACTGGGGAAGTGATTGATTCATAGGGTTTATGATATCTAAAGCTATCACGTTGAAAGTGAAAGTGAAGTCGCTCAGTCGTGTCCAACTCTTTTCAACCCATGGACTCTAGCCCACCAAGCTCCTCCGTCCATGGGATTCTCCAGGCAACAATACTGGAGTGGGTTGCCATTTCCTTCTCCAGGGGATCTCCCTGACCCAGGGATCGAACCCAGGTCTCCCACATTGCAGGCAGGCGCTTTAACCTCTGCGCCACCAGGGAAGCCCTTAAATACAAGAATCAGAGATTTCAAGGGAGGAAAGGAAGCCAGGTTGAAAGAAGAAGCGGGAGGAAGCAGGAAAGGGAGGCGAAAGGGGTGGCCTTATCACTTTGAATGGCAACTAACTTGCCTTTTCCAGTAGGCTATAATCCGGCCACTGTATTACATACACCATCTTACCTCCATGTCACGGTGGTATGTTTAGGCCTGGTGTTTCTTTAAATACAACTGTCTAATCAGATATTTTTCCCTCTCCCCAGTTCTGAGAAGAAATTCAACTGCTTAGATTAAAAAGGAAAGAATAAGGACGGTTGCTCTTATCGCCACTGAAAATGTACCCTTACTCAGAAGCTGGTAAAAGCTGAGCGTATCTACTTTGATTTTTTGTTTGTGTGCTTGTTTGTTTCAG
Seq C2 exon
GACTGCTTATAGGCGAGATCATGTCTGCAGTCCTGAGTCAGGAAGGCATTGATAGTCTTACCCGCCTCCCCAAAGGCAACGCAGAGGCGGAGCTGATGAGCGTGGTCCCAGTGTTCTATGTTTATCACTACCTGGAGGCGGGACATAATTGGGACATCTTTTCTACGAACTCATTAACTCAAAAACAGAACCTGAAGACAAAATTAAAAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000012210:ENSBTAT00000016204:23
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF105694=Thiol-ester_cl=WD(100=43.1),PF076789=A2M_comp=PU(0.4=1.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCATCATTAGCAGGCGAAAGG
R:
ATTTTGTCTTCAGGTTCTGTTTTTGA
Band lengths:
294-1620
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]