BtaINT0054760 @ bosTau6
Intron Retention
Gene
ENSBTAG00000025410 | EGFL8
Description
Bos taurus EGF-like-domain, multiple 8 (EGFL8), mRNA. [Source:RefSeq mRNA;Acc:NM_001099187]
Coordinates
chr23:27026235-27026647:-
Coord C1 exon
chr23:27026521-27026647
Coord A exon
chr23:27026358-27026520
Coord C2 exon
chr23:27026235-27026357
Length
163 bp
Sequences
Splice sites
5' ss Seq
GAGGTGACA
5' ss Score
3.85
3' ss Seq
TTTCTCCCACATGGGTGCAGTCA
3' ss Score
4.49
Exon sequences
Seq C1 exon
CTGGCAGTCTGAAGTCAAGGAGAATCATGGGGTCCAGGGCTGAGCCGTGCACTGTCTTAGGCGGACTCTGGTTCCTCCTGCTACTGATGACAGGCGAGGGGGCCAAGGGTGGAGCCCTCAAAGAGAG
Seq A exon
GTGACAACAGAGGTGGCAGGGCCAGGGATGGGGCTTCACAGGGGGCAAAACAAAGTTGAGGGGGGGATGAGGCTGTCATGTTTAGGGAGGGTGAAGGGAAGTGGGGCTCTGGCTCCCAGGGCCTGGCCCACTCAGTGTCTCACTTTCTCCCACATGGGTGCAG
Seq C2 exon
TCAGGGGGTCTGCTCCAAGCAGACCCTGGTGGTCCCACTCCGTTACAATGAGTCCTACAGCCAACCTGTATATAAGCCCTATCTGACCCTGTGCTCTGGAAGGCGTGTCTGCAGCACCTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000025410:ENSBTAT00000005817:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF075468=EMI=PU(0.1=0.0)
A:
NA
C2:
PF075468=EMI=PU(57.7=97.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Chicken
(galGal4)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGCAGTCTGAAGTCAAGGAGA
R:
CTGTAGGTGCTGCAGACACG
Band lengths:
249-412
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]