RnoINT0053397 @ rn6
Intron Retention
Gene
ENSRNOG00000000436 | Egfl8
Description
EGF-like-domain, multiple 8 [Source:RGD Symbol;Acc:1549706]
Coordinates
chr20:4379689-4380084:-
Coord C1 exon
chr20:4379987-4380084
Coord A exon
chr20:4379812-4379986
Coord C2 exon
chr20:4379689-4379811
Length
175 bp
Sequences
Splice sites
5' ss Seq
AAGGTGACA
5' ss Score
5.19
3' ss Seq
CCCCGCTGGTGTCCCTGCAGTTT
3' ss Score
9.17
Exon sequences
Seq C1 exon
ATGGAGCCCTGGGCTGGGTGCTGCATTTTCCTACGAGGATTCTCATTCCTCCTGGTACTGATGACAGAAGGGGCCAAAGGAGGACCTTTCAAAGAAAG
Seq A exon
GTGACAGCCAGCGGGGGTGGGGGTATCTTTGGGCAAAAGAATGGAAAGTTCTGAATGAAGGCAAGAGACCTGGGTGGGGTGGGGGGATGGGAGGGGGTGCCTGGTCTCGATTGTGAGTTGCTGAGGGCCAGACACACTCCCTGTCTTCTACCTTCCCCCGCTGGTGTCCCTGCAG
Seq C2 exon
TTTGGGAGTGTGCTCCAAGCAGACGCTGCTGGTTCCACTCCGTTACAATGAGTCCTACAGTCAACCAGTGTACAGACCCTACCTGACCCTGTGTGCTGGAAGGCGCATATGTAGCACCTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000000436:ENSRNOT00000000499:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF075468=EMI=PU(0.1=0.0)
A:
NA
C2:
PF075468=EMI=PU(57.7=97.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGCTGGGTGCTGCATTTTC
R:
TGTAGGTGCTACATATGCGCC
Band lengths:
210-385
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]