MmuINT0055820 @ mm10
Intron Retention
Gene
ENSMUSG00000015467 | Egfl8
Description
EGF-like domain 8 [Source:MGI Symbol;Acc:MGI:1932094]
Coordinates
chr17:34614879-34615286:-
Coord C1 exon
chr17:34615167-34615286
Coord A exon
chr17:34615002-34615166
Coord C2 exon
chr17:34614879-34615001
Length
165 bp
Sequences
Splice sites
5' ss Seq
GAGGTGACA
5' ss Score
3.85
3' ss Seq
CCTCCCTGGTGTGCCTACAGTTT
3' ss Score
7.96
Exon sequences
Seq C1 exon
CCCTCAGAGTGAGGGAGCCATGGGGCTCTGGGCTGAGCTGTGCATTTCCCTACGAGGACTCTCATTCTTCCTGGTGCTGATGACGGGCGAAGGGACCAGAGGAGGATCTTTCAAAGAGAG
Seq A exon
GTGACAGCCAATGGGGAGGGCTTTGCTGGGATGGGGTTTGCCATGGGACAGAAAATTGAAAGTTCAGAATGAAGGCGAGATGCCAGGGTGGGAGGGGGGCGCCTGGTCTCAGCTGTGTGAGGTGCTGAGGGCCCGACACCCTCCTCCTCCCTGGTGTGCCTACAG
Seq C2 exon
TTTGGGAGTGTGCTCCAAGCAGACGCTGCTGGTTCCTCTCCGTTACAACGAGTCCTATAGTCAACCGGTGTACAAACCCTACCTGACCTTGTGTGCGGGGAGGCGCATATGTAGCACCTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000015467:ENSMUST00000015611:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF075468=EMI=PU(0.1=0.0)
A:
NA
C2:
PF075468=EMI=PU(57.7=97.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCAGAGTGAGGGAGCCATGG
R:
TGTAGGTGCTACATATGCGCC
Band lengths:
240-405
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types