BtaINT0054764 @ bosTau6
Intron Retention
Gene
ENSBTAG00000025410 | EGFL8
Description
Bos taurus EGF-like-domain, multiple 8 (EGFL8), mRNA. [Source:RefSeq mRNA;Acc:NM_001099187]
Coordinates
chr23:27025385-27025710:-
Coord C1 exon
chr23:27025540-27025710
Coord A exon
chr23:27025465-27025539
Coord C2 exon
chr23:27025385-27025464
Length
75 bp
Sequences
Splice sites
5' ss Seq
CCGGTGAGT
5' ss Score
10.9
3' ss Seq
TCCCTCTCCTTGGTCCCCAGTTC
3' ss Score
9.02
Exon sequences
Seq C1 exon
ATGTGGATGAATGCAGAACCGGCGTCACTCTCTGCTCGCATCGCTGCCTCAATACGGCGGGCAGTTTCACCTGTGGCTGCCCGCACGGCCTGGTGCTGGGCCTGGATGGGCGTACTTGCGCGGAGAGGGCCCCAGAGCCCCCAACCAGTGCCAGCATCCTCAATGTGGCCG
Seq A exon
GTGAGTCGGTGGGAGCAGGGGGCGAATAGAGGCAGGGCCGGGGCAGCAGTGACGCTCCCTCTCCTTGGTCCCCAG
Seq C2 exon
TTCGTGAGGCTGGACTTGATAAGCGTGCCCTGAGGCGGGAGATTGGCGAGCTGCGAGGCCGCCTGGAGAGGCTGGAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000025410:ENSBTAT00000005817:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.052 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0764510=EGF_CA=WD(100=69.0)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCGCATCGCTGCCTCAATAC
R:
CCTCAGGGCACGCTTATCAAG
Band lengths:
172-247
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]