RnoINT0053401 @ rn6
Intron Retention
Gene
ENSRNOG00000000436 | Egfl8
Description
EGF-like-domain, multiple 8 [Source:RGD Symbol;Acc:1549706]
Coordinates
chr20:4378836-4379167:-
Coord C1 exon
chr20:4378997-4379167
Coord A exon
chr20:4378916-4378996
Coord C2 exon
chr20:4378836-4378915
Length
81 bp
Sequences
Splice sites
5' ss Seq
CAGGTGGGT
5' ss Score
8.56
3' ss Seq
TCTGTTCTCCTTTGCCCTAGTTC
3' ss Score
9.41
Exon sequences
Seq C1 exon
ATGTGGATGAATGCAGGACCAGCCTTACCCTCTGCTCTCATGGCTGTCTCAACACGCTGGGCAGCTTCACCTGCAGCTGTCCGAACCCCCTGGTGCTGGGGCCCGATGGACGCGCCTGTGCAGGGGGCCCTCCGGAGTCCTCGACGAGTGCTGGCATCCTCAGTGTGGCAG
Seq A exon
GTGGGTGTGGGGCACCTGTGGGGTGGCCTGGTCTCTGCAGAGGCGCCGGGGAAGGCCACGCTCTGTTCTCCTTTGCCCTAG
Seq C2 exon
TTCGGGAAGCAGACAGCGAGGAGCATGCCCTGAGGCGGGAGGTTGCAGAGCTTCGTGGGCGTCTAGAGAGGCTGGAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000000436:ENSRNOT00000000499:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.129 A=NA C2=0.037
Domain overlap (PFAM):
C1:
PF0764510=EGF_CA=WD(100=69.0)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTGCTCTCATGGCTGTCTCA
R:
ATGCTCCTCGCTGTCTGCTTC
Band lengths:
167-248
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]