BtaINT0063751 @ bosTau6
Intron Retention
Gene
ENSBTAG00000008994 | FPGS
Description
Bos taurus folylpolyglutamate synthase (FPGS), mRNA. [Source:RefSeq mRNA;Acc:NM_001024480]
Coordinates
chr11:98502921-98503264:+
Coord C1 exon
chr11:98502921-98503035
Coord A exon
chr11:98503036-98503183
Coord C2 exon
chr11:98503184-98503264
Length
148 bp
Sequences
Splice sites
5' ss Seq
AAGGTGCCT
5' ss Score
5.39
3' ss Seq
TGACATGATCCTGTCCACAGGAC
3' ss Score
10.22
Exon sequences
Seq C1 exon
CTCTCCCCACCTGGTGCAGGTGCGTGAGCGGATCCGCATCAATGGGCAGCCCATAAGCTCCGAGCTCTTCACCAAGCACTTCTGGCGCCTCTACCACCGGCTGGAGGAGACCAAG
Seq A exon
GTGCCTGTGCCAGAGGGCTGGTGGGTGGGCAGGCTAGGGGACCTCAAGTGGGTGGGTAGACCCAGAGGGCGCTGCGCATCCCAGGACACCATCTCCCCCAGCACAGAGGCTGCAGTGGCCCCGAGCTCTGACATGATCCTGTCCACAG
Seq C2 exon
GACGACAGCAGCTGTGTCTCCATGCCCGGCTACTTCCGCTTCCTCACGCTCATGGCCTTCCACGTCTTCCTCCAAGAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000008994:ENSBTAT00000032762:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF082457=Mur_ligase_M=FE(17.8=100)
A:
NA
C2:
PF082457=Mur_ligase_M=FE(12.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCTCCCCACCTGGTGCAG
R:
TCTCTTGGAGGAAGACGTGGA
Band lengths:
194-342
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development