BtaINT0068789 @ bosTau6
Intron Retention
Gene
ENSBTAG00000021658 | GRASP
Description
GRP1 (general receptor for phosphoinositides 1)-associated scaffold protein [Source:HGNC Symbol;Acc:HGNC:18707]
Coordinates
chr5:28023507-28023770:-
Coord C1 exon
chr5:28023716-28023770
Coord A exon
chr5:28023610-28023715
Coord C2 exon
chr5:28023507-28023609
Length
106 bp
Sequences
Splice sites
5' ss Seq
CAGGTGGGA
5' ss Score
6.71
3' ss Seq
GGTCCCCTTGCCTGTTACAGACG
3' ss Score
7.3
Exon sequences
Seq C1 exon
GAAAGTGCTGACTTTGGAGAAGGAGGAGAACCAGACCTTTGGCTTTGAGATCCAG
Seq A exon
GTGGGAGAGAAGCTGGGTGCAGAGGGAGGGAAGGGTTGGGAGGCAGTCTGAGGGACGGGTGGATCCTGCTCCACCCCTGGGCTGAGGGTCCCCTTGCCTGTTACAG
Seq C2 exon
ACGTATGGCCTTCACCATCGAGAGGAGCAGCGTGTGGAGATGGTGACCTTTGTCTGCCGGGTTCATGAGTCCAGCCCTGCCCAGCTGGCAGGGCTCACACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021658:ENSBTAT00000028862:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.526 A=NA C2=0.086
Domain overlap (PFAM):
C1:
PF0059519=PDZ=PU(18.4=84.2)
A:
NA
C2:
PF0059519=PDZ=FE(39.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGCTGACTTTGGAGAAGGAGG
R:
CTGGTGTGAGCCCTGCCAG
Band lengths:
154-260
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]