BtaINT0070153 @ bosTau6
Intron Retention
Gene
ENSBTAG00000000704 | GUSB
Description
Bos taurus glucuronidase, beta (GUSB), mRNA. [Source:RefSeq mRNA;Acc:NM_001083436]
Coordinates
chr25:28170322-28170970:-
Coord C1 exon
chr25:28170792-28170970
Coord A exon
chr25:28170469-28170791
Coord C2 exon
chr25:28170322-28170468
Length
323 bp
Sequences
Splice sites
5' ss Seq
GGTGTGAGT
5' ss Score
6.05
3' ss Seq
TCTCCTCTCTTGGCCTGCAGCGA
3' ss Score
10.17
Exon sequences
Seq C1 exon
ATCCGAGGGAAGGGCTTTGACTGGCCGCTGCTGGTGAAGGACTTCAACCTGCTTCGCTGGCTGGGCGCCAACGCTTTCCGCACCAGCCACTACCCCTATTCAGAGGAGGTGCTGCAGCTCTGTGACCGCTACGGGATCGTGGTCATCGACGAGAGCCCTGGTGTGGGCATCGTGCTGGT
Seq A exon
GTGAGTGCCCGCTGCCTGCACTGCGCGGCACCCCCAGTTCGGCAGCCTGTGCGTGCATGCTGTCGCTCAGTCATGTCTGACTCTGGGACCCTATGGACTGTAGCCCATCAGACTCCTCTATCTATGGGATTCTCCAGGCCAGAATACCAGAGTGGGTTGCCATTTTCCTTCTTCAGGGGATCTTCCCGACCCAAGGATCGAACTTGTGTTTCCTACGTTGGGAGGCGGGTTGTTTACCACTGAGTCATCAGGGAAGCCCAGAGTTTCTACTAGAGGGAAAGAAAATCAGACTGACCTGTTCCCTCTCCTCTCTTGGCCTGCAG
Seq C2 exon
CGAGAGCTTCAGCAACGTGTCTCTGCAACACCACCTGGAGGTGATGGAGGAAATGATCCGCAGGGACAAGAATCACCCGGCCGTTGTAATGTGGTCCTTGGCCAATGAGCCCGCTTCCTTCCTGAAACCGGCTGGTTACTACTTCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000000704:ENSBTAT00000000941:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0283612=Glyco_hydro_2_C=FE(19.5=100)
A:
NA
C2:
PF0283612=Glyco_hydro_2_C=FE(16.2=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCCGAGGGAAGGGCTTTGA
R:
TTGAAGTAGTAACCAGCCGG
Band lengths:
326-649
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]