BtaINT0072324 @ bosTau6
Intron Retention
Gene
ENSBTAG00000014898 | HK3
Description
hexokinase 3 (white cell) [Source:HGNC Symbol;Acc:HGNC:4925]
Coordinates
chr7:39701215-39702094:-
Coord C1 exon
chr7:39701861-39702094
Coord A exon
chr7:39701511-39701860
Coord C2 exon
chr7:39701215-39701510
Length
350 bp
Sequences
Splice sites
5' ss Seq
CAGGTACTT
5' ss Score
8.17
3' ss Seq
CCTGCTGTGTGTCTCTGTAGGTT
3' ss Score
12.21
Exon sequences
Seq C1 exon
TCCCTCTGCTGGGGCAGCCCGTGTGCACGCTGTCCTGCAGGACTTGGGCCTGAACCCGAAGGCCTCAGATGCTGAGTGGGTGCAGTGTGTGTGCATGGCTGTGTGCACGCGGGCCGCCCAGCTCTGCGCTGCTGCTCTGGCCGCTGTCCTCTCCCGCCTCCAGCACAGCCGGGAGCAGCAGGCTCTTCAGATTGCTGTGGCCACCGGAGGCCGAGTGTTTGAGCGACACCCCAG
Seq A exon
GTACTTGGAATACAGCATGAACCTGTGTGAACAGGTCACCAGAGCATGCGTGTGTGTGTGATACCAGAGGGCACTCTGGTACCACAGGTGCAAGGCAGTGGGTGTGAATGGCTCCCTAGCAGAATAAAGATGGACGGAGGGTTAAGGGAGGCCAGAAGAGGTCAAAGGAGAGAAAGAGGGTTCTCTTGGGTGTGCAAGGGAGGGGATCACAGGTGCACTGGGTGCATGGCTGGTGCACAGATGGGTTTAGAACTGAACTGATTTGAAATGAGGGAGCCCAGGGCTTCAGCATGCTGCGGGGGTCAGGGCCAGGTGCCAAGCCAGGGCGCACCTGCTGTGTGTCTCTGTAG
Seq C2 exon
GTTTCTCAGTGTCCTACGGGAGACAGTGATGCTTCTGGCCCCCAACTGCGATGTCTCCTTCATCCCCTCTGTGGATGGGGGTGGCCGGGGCGTGGCAATGGTGACTGCTGTTGCTGCCCGCCTGGCTGCCCACCGGCGCCTGTTGGAGGAGACCTTGGCACCATTCCGGTTGACCCGGGAGCAGCTGGCAGAAGTTCAGGCACAGATGCGAGAGGCCATGGCCAAGGGGCTCCAAGGGGAAGCCTCCTCCCTCCGCATGCTGCCCACTTATGTGCGGGCCACGCCTGATGGCAGCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000014898:ENSBTAT00000019834:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0372711=Hexokinase_2=FE(32.5=100)
A:
NA
C2:
PF0372711=Hexokinase_2=PD(16.7=40.0),PF0034916=Hexokinase_1=PU(28.5=57.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTGTGTGTGCATGGCTGTG
R:
ATCTGTGCCTGAACTTCTGCC
Band lengths:
358-708
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]