BtaINT0091450 @ bosTau6
Intron Retention
Gene
ENSBTAG00000047217 | MED16
Description
mediator complex subunit 16 [Source:HGNC Symbol;Acc:HGNC:17556]
Coordinates
chr7:45038435-45039946:-
Coord C1 exon
chr7:45039730-45039946
Coord A exon
chr7:45038519-45039729
Coord C2 exon
chr7:45038435-45038518
Length
1211 bp
Sequences
Splice sites
5' ss Seq
CAGGTATGC
5' ss Score
9.37
3' ss Seq
CCCCTGCTCCTCTCCATCAGGGC
3' ss Score
10.07
Exon sequences
Seq C1 exon
GTCGTGACGAGGGTCCCACAAGTGAGCCAGACGAGGCGCTGGTGGATGAGTGCTGCCTACTGCCCAGCCAGCTGCTCATCCCCAGCCTGGACTGGCTGCCTGTTAGTGACGGACTGGTCAGCCGCCTGCAGCCCAAGCAGCCCCTGCGCCTGCACTTTGGCAAGGCCCCTGCTCTTCCTGGTAGCTCCACTGGTCTGCAGCTGGATGGCCTCATCAG
Seq A exon
GTATGCGCACTCACCTCTGACTCAGGGCCCTGGATACCTTTTCTTTCAGAAGAGTCTCCCTTAGACCTGCCTGACATTCCCTGTCACATGTCTGTCTCAGGGCCTCTGCCCATGCTGCGCCTGCTGCTTCCTTCCAGCCTGACCTGAGTGCTACCCCCGATACTCCTCCCCTCCCTTGCCCCTGAGATTCCTCCTTGCCCCCAGACTCCTCCCCGCCCCACCCACCTCCCCTGCCCTGTCACTTCCCTCTATCCAGACACTATACTGTACTATGGTGTACAGGTCATCGGCTTGTACTAATTTGTGTCCTTGCTTGTCCGGGTGTCGTCATCTTCCCCCTAGCCTGTCACGGCCCGCATGGAGGGCCCACTTGGCCAACCTTGGTTACTCTGGAGCTCAGGTTCACACCCCAAGCCCCCACCATAAATCGCGCTGCAGCCCAGAGTGCCTGGCAGGTCCCAAGGTGCCAGGAAGCAGAACACTCTTAGGCAGACCTTGCTGGGAGTGACCTCGGGGGCCATTCTTTGGATGTACGGAGTCCAGAAAGCCCAGGCCTGCTGAGGGAGCAGCACCCTCGGTGGGGGTTTGTCCCCACTTTACAGGCAGGTAATCTCAATCTCCAAGGGACAGTCCAGAGATAGACGCCAGGCCAGAAGGCGAACCTAGGACTCCACAGGCCACCTCCCATTCAGGGTGTCATGGGATGGATTCTCAAGTGGTCCTTTACTGGGGAGGGGCCTTAGCATCCCCACAGCCCCCTTCCCCTCCGGGAAGACTCTGGGCCTTCCTCCTCCACTTCCGGATTTTCTTTCTCATCTCTAAAGCCGTGGGAGGAACGGCTTGATGAATCACACACTTGTGGTTTGTGCTCGAGTTTCAGGAAACACTTTACACAGAGAGGCGGCTGGTCGCCAAAGGGCTTTCACACTCTAGGAAGTTTTCAAACTTGTGTGATACGAGTGCCCCAGCCTCAGAAATCCTGATGGGCTCGCTCCACTCTGCTCCAGGCGTCACCCTCAGCAGGGGCCTGTGGGCAGAGGGTGTGTAGGAGTCTGGCTCCTGGATTCTCCGTGTTCTCACACACGCTTTGCCTCTAGGAGATTGACCCGTACGTCTGTATGTGCGCATGTGCTTGTGTCTGCGTGTGTGCATGCATGTGTGCGCCCATGCATGTGCACACGCCCGAGCCTTCCCCTGCTCCTCTCCATCAG
Seq C2 exon
GGCGCCGGGCCAGCCCAAGATGGACCACCTGCGGAGGCTGCACCTGGGCGCCTACCCCACGGAGGCCTGCAAGGCGTGTACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000047217:ENSBTAT00000064681:15
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF116353=Med16=FE(10.0=100)
A:
NA
C2:
PF116353=Med16=FE(3.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACGAGGGTCCCACAAGTGAG
R:
CTGGTACACGCCTTGCAGG
Band lengths:
296-1507
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]