BtaINT0094528 @ bosTau6
Intron Retention
Gene
ENSBTAG00000003922 | MPHOSPH8
Description
M-phase phosphoprotein 8 [Source:HGNC Symbol;Acc:HGNC:29810]
Coordinates
chr12:36611240-36613206:-
Coord C1 exon
chr12:36613052-36613206
Coord A exon
chr12:36611395-36613051
Coord C2 exon
chr12:36611240-36611394
Length
1657 bp
Sequences
Splice sites
5' ss Seq
GACGTAAGT
5' ss Score
10.93
3' ss Seq
TTACTCTGTTTTTTTATTAGACT
3' ss Score
8.51
Exon sequences
Seq C1 exon
GCTTGTAAAAGAGGAAACTCAGACATTGTCCGGCTGGTCATTGAATGTGGAGCTGACTGCAATATTCTGTCAAAGCACCAGAACAGTGCGCTGCATTTTGCAAAGCAGTGCAACAACGTGCTGGTGTATGACCTGCTGAAGAGCCACTTAGAGAC
Seq A exon
GTAAGTGTGGGAGGAGTGTGCCCTGGATGTAATGGACAGTGTGTATGTAACAGCTCAGAAAAAGATAAGTACAAGTCACAATGGGTTTCTGGTCTAAGAAGATGAATTTTAAAAGTGGTGTACAAAGTCTGCAAGTTATTGAGGACTTAGAAACCTTCAAACTTAATGCTGTTCTATCTAGGAGTATCATCTTAAAATATTAATTACACTCTTAACTTGGCTTCCAAACATTTTCTTAAAGCCATTTAAACTTCATTGTTTCTAAGAGATACAATTTTTGAGAGCAGTTTTTAGATTCTGAGCGAAGTTGAAAAGAAAGTAGCGATTTCCCACATGCATTGTCCTACCCTGGCAGTGAGGCTGTTACAGTCAATGGACCCACCATCATGGCTGACCCGCACATCCCCCTGACCTAGAGCCCCCCGAGGTTCCCCTTGGTGTCACAGATCCTGTTTTCGGGCGAATGTGTGATGACGTGTACCCACCATCGCCCTGTCACACACTCAACTCACCACCCTAAACATCCTCTGTGCTTCCCCATCTGTTCAACTTGCCCAAACCCTGGAGACCACTGATGTTTTTACTGCTAGTTGGACTCATACACTATTTGCCCTTTTCCAGGTTTGCATCTTTGTGATAAGCATTCATGTCTCCCCATCTTTACGCAGCCTGATAAACTTGTTTTAGTGCTGAATGGTTATTACATTGTCTGGAAATTATCACAGTTTAGTTATTCAAGTTCCTGCCTTTTCAAATGAATTCAAAATCATCAGATGTTATTGGAAGTCCATAAAAGTAATCTAGTAGTTTTTTATTACTTCTCTTGCTCACATGTAACAGGTGCTTCAGGAGCAGTACTGTTGTAGGTAAGGGGCTTTCTGTCAGGTAGCAGAGCCCCTCTCCTCCCCACCCACCAAGGGTAAGATAGGACTTGGATCTTCACACCTGAAAGATGGTTATTCTCATTCCAGATATAAGCAAATTTCAGACAAGAGACAAAAAAAAAAGGCAAAAGGGGGTGTTTCTAGCAACCTGGGCTGCCATCCCTGAGAGCCTCAGCAGCCAGGTATTGGTTATAGGTGGCCACCAAGTAGAGAGACTAGCCTGCCTGCTTTGCCCACAGCTGAGGGGGGGTCCGGGCATTGCTGGGCTTTGTTTCACATCTGTGAGTAAATGGTTTTGGAGATTTTATGCTCCTTAAAGCAGAGCATCTTTTGGAAGGTGGCAGGGGGGGTTCGGGTCAGATCAGTTACACTTTACCTGCAGGATAGTCTCAAAATACTTTTCTGAATTAGCACCAATCATTTTATTTGACTGCTTTCTAAACCAAGTACTATAACAAGACTTTGTGTGACAATTCTTGGCTTTATTTTCTAGCAGAATTCTTATTTCTGATTCTCCTTGATCATTCATACACTCACTTGACGAGTGTGTGTTCAACTCCAACTGTGTGCCTGGCACAACGCAAGAGACTCAGGGGGTGGGGCGTGAGAGGACATCTAAGACAGACAGGTGTGTGCTGAGCACACAGGAGTGCTCAGAACAAAGAGCAAGGGGCAGGAGTTTTTCAAGATGGCTCTGGGGAGGCTTCACTAGTCCTACTGTTTTGTGCCTCAAGATTCTTACATGAACAGCGTTTACTCTGTTTTTTTATTAG
Seq C2 exon
ACTTTCGAGAGTAGCTGAAGAAACTATCAAGGATTACTTTGAAGCACGTCTTGCGCTACTGGAACCAGTCTTCCCAATAGCGTGCCATCGCCTCTGTGAGGGGCCAGATTTCTCAATGGATTTCAATTACAGACCCCCACAGACTATACCAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000003922:ENSBTAT00000061268:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.047
Domain overlap (PFAM):
C1:
PF127962=Ank_2=FE(54.8=100)
A:
NA
C2:
PF127962=Ank_2=PD(5.4=9.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACTCAGACATTGTCCGGCTG
R:
TTCTGGTATAGTCTGTGGGGGT
Band lengths:
294-1951
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development