BtaINT0130798 @ bosTau6
Intron Retention
Gene
ENSBTAG00000012228 | SEMA4A
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [Source:HGNC Symbol;Acc:HGNC:10729]
Coordinates
chr3:14675415-14675855:-
Coord C1 exon
chr3:14675793-14675855
Coord A exon
chr3:14675514-14675792
Coord C2 exon
chr3:14675415-14675513
Length
279 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAGT
5' ss Score
11.08
3' ss Seq
GAACTGTCTCTCTCCCCCAGACA
3' ss Score
10.7
Exon sequences
Seq C1 exon
ATACCCTGGCCAGCCAGTGACAGAAAAAAGAGTGAATGTGCCTTTAAGAGGAAGAGCAATGAG
Seq A exon
GTAAGTAGGGGTGGCGATACTGGGGCTCTGCAAGAGAGCCCTGGTGTCCCCAGGCCATCACTGGATGACCTTAGGTGAGGCAGGCAGCAGAGGATGCTTGTTTGAGTGATCGTGTTCTAGAGGATCATGATCACGTGGCCTGCTGTTCTATAAAGACCTATGGGGACTGCCTCAGCACTGTCCAGCCCTTCTCCCTCCTGGCAAGTGAGGGAGCAGTCCTCCAGGGGCCGGGAGTCTGGGGCAAGTGTTCTGGAGGCCTGAACTGTCTCTCTCCCCCAG
Seq C2 exon
ACACAGTGTTTCAACTTTATTCGTGTCCTGGTATCCTTCAACTCCACCCACCTCTACGCCTGCGGCACGTTTGCCTTCAGCCCTGCTTGTACCTTCATT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000012228:ENSBTAT00000016223:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(4.8=100)
A:
NA
C2:
PF0140314=Sema=FE(7.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGCCAGCCAGTGACAGAAAA
R:
AATGAAGGTACAAGCAGGGCTG
Band lengths:
156-435
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]