BtaINT0139378 @ bosTau6
Intron Retention
Gene
ENSBTAG00000032544 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr14:66380010-66381106:-
Coord C1 exon
chr14:66381000-66381106
Coord A exon
chr14:66380116-66380999
Coord C2 exon
chr14:66380010-66380115
Length
884 bp
Sequences
Splice sites
5' ss Seq
CAGGTAATG
5' ss Score
9.43
3' ss Seq
CAGATAACATTTATTTATAGGTC
3' ss Score
5.43
Exon sequences
Seq C1 exon
ATTTGACGTGGAAAAAGAATGTGCAAAACTTGATGAAGATAAAGAAAGAACTGTAATAAACAACAAATCACATTTGTCTAAAATTGAGACAAGACTAGATACAGCAG
Seq A exon
GTAATGGGGGGAAAATAGGAATGATTTAGTAGTCTTTACTGGAGAAGGCAATGGCACCCCACTCCAGTACTCTTGCCTGGAAAATCCCATGGATGGGGGAGCCTGGTAGGCTGCAGACCATGGGGTCGCTAAGGGTCGGACATGACTGAGTGACTTCACTTTCACTTTTCACTTTCATGCATTGGAGGAGGAAATGGCAACCCACTCCAGTGTTCTTGCCTTGAGAGTCCCAGGGACGGGGGAGCCTGGTGGGCTGCCATCTATGGAGTCACACAGAGTCGGACATGACTGAAGGGACTTAGCAGCAGCAGCAGCAGTAATCTTTACCTTTTTTGAATGATAATAAAAGCTGTTGACCCAAAGACATTCTCTTTAAGAGCTGTATATTTCCATAAAAAGTTTATGAATTTTCTCCTAGGGCTGATCACCTCTAACAAATTCCATTCTATCCTTAAACTTCTTAATTTTATGAGTAATAATAGTTATGATACTTAAGTGTAATAAGACTTAAAAACATATGAATATGTAAACCTATTTTGTTTTTACCATGATTCAGTTTTCATTCCCTTTTTCAAATCTTTTTTGTCCAAATCCTTCAAATTTCTTTTCCTCAAAACAAAAAGATGGAGCACTTTCTGTTCACTTGAAGTCCACAGGTACATAGTACCTTTAAAGTAAAGCAAAACAATTATTGAGAAATCTAAACCTTCAAAAATTCTGAATGATTAAAAAGAATAGAAATATCATAAACTTAGGAAACCTTTTATTTGCTTTATTTGTTTTTATAGTTTTCTGCTTCTGTTAAAATAAGCATTTTTGGATTTAAATTTGGAAAATTCTATCAAGTTATATTCTTACTTACAGCAGATAACATTTATTTATAG
Seq C2 exon
GTCTAACTGAAAAGGAAAAGGTTTTTATGGCTACGCGTGAAAAGGAAAAAGGAAATGAAGCTTTTAAGTCAGGAGATTATGAAGAAGCTGTAAAGTACTATACTCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000032544:ENSBTAT00000046176:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.189 A=NA C2=0.333
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF134141=TPR_11=PU(39.4=72.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGACGTGGAAAAAGAATGTGCA
R:
CGAGTATAGTACTTTACAGCTTCTTC
Band lengths:
210-1094
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development