HsaINT0157149 @ hg38
Intron Retention
Gene
ENSG00000104450 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr8:100183956-100184733:+
Coord C1 exon
chr8:100183956-100184062
Coord A exon
chr8:100184063-100184627
Coord C2 exon
chr8:100184628-100184733
Length
565 bp
Sequences
Splice sites
5' ss Seq
CAGGTAATT
5' ss Score
8.55
3' ss Seq
CAGATAACATTTATTTCTAGGTC
3' ss Score
6.3
Exon sequences
Seq C1 exon
ATTTGACGTGGAGAAGGAATGTTTAAAAATTGATGAAGATTACAAAGAAAAGACGGTAATAGACAAGTCACACTTGTCTAAAATTGAGACAAGAATAGATACAGCAG
Seq A exon
GTAATTGGAGAAAAAATAATAATTTAGTAGTCTTTAAAGTTTTTCAATTATAATAAAAAGTGTCAATCTAGAAGACATTGTCTTTGAGTACTGCACAGATTACCATGAAAAGTTTATGGATTTTCTCTTACAGTTGACCACTCAACAAATTCACTTCTCTCTTTTACCCACTTAATTTTTAAATGTAGTAAGGCTTTAAAATATAAACCTATTTTTCTTACTGTAATTCATTTGTTTTTAATTCCTTTTTTCCAATCCTCTTTTTCCTCCAAATTCCTAAAATTGTTTTCTTTTTGCTTCAAAACAAAAAGATGGGGCAGTTTATTGGACTAGCACCTATAAAGTGAAACGAAATCAATCATTAAAAAAATTCTGAATCCTCAAAACTTGTGAATGATTTAAAAAGAAAACATCCCTAAGCTTGGGGAACCTTTTATTTTCTTTTGTTTATTTTATGATCTTTCTGCTTTCTTCTGCTTCTGTTAAAATAAGCATTCTTAGATTTAAACTTGAAAAATTCTGTTAAATTATACGTTTACATATAGCAGATAACATTTATTTCTAG
Seq C2 exon
GTCTAACTGAGAAAGAAAAGGATTTTCTTGCCACTCGTGAAAAGGAGAAAGGAAATGAAGCTTTCAACTCAGGAGATTATGAAGAAGCAGTGATGTATTATACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSG00000104450:ENST00000388798:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.050 A=NA C2=0.329
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF134141=TPR_11=PU(39.4=72.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGACGTGGAGAAGGAATGTTT
R:
TGGTATAATACATCACTGCTTCTTCA
Band lengths:
209-774
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Autistic and control brains