BtaINT0155293 @ bosTau6
Intron Retention
Gene
ENSBTAG00000006969 | TUBB5
Description
Bos taurus tubulin, beta class I (TUBB), mRNA. [Source:RefSeq mRNA;Acc:NM_001046549]
Coordinates
chr23:28103241-28103717:-
Coord C1 exon
chr23:28103609-28103717
Coord A exon
chr23:28103352-28103608
Coord C2 exon
chr23:28103241-28103351
Length
257 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGG
5' ss Score
10.07
3' ss Seq
TTTTACTTTGTTTCTTCTAGGTG
3' ss Score
12.68
Exon sequences
Seq C1 exon
TTCTGGGAGGTGATCAGTGATGAACATGGCATCGACCCCACCGGCACCTATCATGGGGACAGCGACCTACAACTGGACCGCATCTCCGTGTACTACAATGAAGCCACAG
Seq A exon
GTGAGGGCAAGAGCTCGGGAAGCAGGGGTCCCATCACTCCCACTTCTCTGGGGTCTCTGCCTCCCCCGGACACCCTTTACCCCCTTAGAATGAATCCATCTACACTCATTGGAGACCTTTCTCGCTTTCCCAGTGGCTCTGCTGCCACCTGGTGGCAGTGTCTGAAATCACACATTTGGGTCCCCGGCTCTCTCTTGGCCCCAATTTATTTACCTTTATGTCTTCCAGTTTTCATTCTTTTACTTTGTTTCTTCTAG
Seq C2 exon
GTGGCAAATATGTCCCTCGTGCTATCTTGGTGGATCTAGAACCCGGAACCATGGACTCTGTTCGCTCAGGTCCTTTTGGTCAGATCTTCAGACCAGACAACTTTGTTTTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000006969:ENSBTAT00000009158:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.135 A=NA C2=0.026
Domain overlap (PFAM):
C1:
PF0009120=Tubulin=FE(16.2=100)
A:
NA
C2:
PF0009120=Tubulin=FE(16.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Chicken
(galGal4)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTCTGGGAGGTGATCAGTGATGA
R:
CAAAAACAAAGTTGTCTGGTCTGA
Band lengths:
220-477
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]