RnoINT0158244 @ rn6
Intron Retention
Gene
ENSRNOG00000061216 | Tubb5
Description
tubulin, beta 5 class I [Source:RGD Symbol;Acc:628596]
Coordinates
chr20:3423986-3424544:+
Coord C1 exon
chr20:3423986-3424094
Coord A exon
chr20:3424095-3424433
Coord C2 exon
chr20:3424434-3424544
Length
339 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGG
5' ss Score
10.07
3' ss Seq
AACATCTTGAATACTTGTAGGTG
3' ss Score
5.38
Exon sequences
Seq C1 exon
TTCTGGGAGGTGATAAGCGATGAACACGGCATCGACCCCACCGGCACCTACCACGGAGACAGCGACTTGCAGCTGGACCGAATCTCTGTGTACTACAATGAAGCTACAG
Seq A exon
GTGAGGGAAGAAATCCCGATTCTTCTGCGTGCCCCCCTATTGAGACAGGGACCCCATTCATCACTGGCCGCCCTGGAACTCACATAGATCGGTCTACCTTCCAAGTGTTGGGTTTAAAGGCAGCATCACCACCCTGTTTTCCCACACTTGGGTATCAGTTACCCCTCTGTGAGACTCCCATTTTTCCCCCCTTACACACACACCTTATGGGGCTGAAGGTTGGCTCTACTGCCACCAGGTGGTAGTGCCTGGAATGACAACTTTAGTGATATGTGGCTGATTTCTCCACCCCAAATTTGAGCTATTTCATCGCTGATCGAACATCTTGAATACTTGTAG
Seq C2 exon
GTGGCAAGTATGTCCCTCGAGCTATCTTAGTGGATCTAGAACCCGGGACTATGGACTCCGTTCGCTCAGGTCCTTTTGGCCAGATCTTCAGACCGGACAACTTTGTTTTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000061216:ENSRNOT00000079854:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.135 A=NA C2=0.026
Domain overlap (PFAM):
C1:
PF0009120=Tubulin=FE(16.2=100)
A:
NA
C2:
PF0009120=Tubulin=FE(16.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTGGGAGGTGATAAGCGATGA
R:
ACAAAGTTGTCCGGTCTGAAGA
Band lengths:
214-553
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]