BtaINT0155282 @ bosTau6
Intron Retention
Gene
ENSBTAG00000004093 | TUBB2B
Description
Tubulin beta-2B chain [Source:UniProtKB/Swiss-Prot;Acc:Q6B856]
Coordinates
chr23:50355742-50356231:+
Coord C1 exon
chr23:50355742-50355850
Coord A exon
chr23:50355851-50356120
Coord C2 exon
chr23:50356121-50356231
Length
270 bp
Sequences
Splice sites
5' ss Seq
CCGGTAATC
5' ss Score
3.57
3' ss Seq
GTCCTGCTCTGTCCCCGCAGGTA
3' ss Score
13.72
Exon sequences
Seq C1 exon
TTTTGGGAAGTCATCAGTGATGAGCATGGCATCGACCCCACTGGCAGTTACCACGGAGACAGTGACTTGCAGCTGGAGAGAATCAATGTGTACTACAATGAGGCCACCG
Seq A exon
GTAATCGCCTTTACCCCGCGCGCCTCCCCAAGGGACTGACCCCAGAAGTGTAGCCCCAGGGCAGGGTGGTTTAGCGGATGTAGGATTCCTATCTTTCCCTGAGGTTTTTTTTTTTGGAGGGTGGAGGGAACGGAGGTGGTGGGGGTGTGAGTGCTCCTTTGAGAATCTTGTTAGAATCCCGTCTTGTGTCCTTTGTTGGGGATACAAGCAGCGCTGCAAGGAAGGAAGCTGGTACCAGGGGCAGGTCTGAGTCCTGCTCTGTCCCCGCAG
Seq C2 exon
GTAACAAGTATGTGCCTCGGGCCATCCTGGTGGACCTGGAGCCGGGCACCATGGACTCCGTCAGGTCTGGACCCTTCGGCCAGATCTTCAGGCCTGACAACTTCGTGTTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000004093:ENSBTAT00000005346:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.135 A=NA C2=0.026
Domain overlap (PFAM):
C1:
PF0009120=Tubulin=FE(16.2=100)
A:
NA
C2:
PF0009120=Tubulin=FE(16.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTTTGGGAAGTCATCAGTGATGA
R:
CAAACACGAAGTTGTCAGGCC
Band lengths:
220-490
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]