DreINT0169766 @ danRer10
Intron Retention
Gene
ENSDARG00000037997 | tubb5
Description
tubulin, beta 5 [Source:ZFIN;Acc:ZDB-GENE-031110-4]
Coordinates
chr16:10431642-10433023:+
Coord C1 exon
chr16:10431642-10431750
Coord A exon
chr16:10431751-10432912
Coord C2 exon
chr16:10432913-10433023
Length
1162 bp
Sequences
Splice sites
5' ss Seq
CAGGTACTT
5' ss Score
8.17
3' ss Seq
TTTCTCTATGTCTCGTGGAGGAG
3' ss Score
0.64
Exon sequences
Seq C1 exon
TTTTGGGAGGTGATCAGCGATGAACATGGAATCGACCCAACCGGAACCTATCACGGAGACAGTGACCTCCAGCTGGACAGAATTAGTGTCTACTACAATGAGGCTACAG
Seq A exon
GTACTTTAAGACATTGGTATAAAGAAAGATGAAAGTATATTATTAGCATGAACAGTATTGATAATCAAAAGTTTTTTTTTTACTTTTTAATGATTGTTGAAAAGATTTGTTACCAATTCTTTATTAGTTATGTCATAACATAAAATGCATTATAATCTTTAAGTTTTAATCTTTAATCAAGCATTTCACTTGGAAGTTCATCCCAATAAAGGAGAAAATATATTTCTATTTCATAATTATTTTTAGAGAAATACATGGAAAGTTTTCAAATAGTTCTTAAAGCAAATGGCTGAAAATCATGAAATGTGACATGAATATGTGATACGAGATCAGTTCTATTTATTTTAATTGATTCATTCAAGTTTTTAAACTTTTCTGAATCTGATTTTTGCTAATTATGTAATATGACTTTAATCATTTTAACGTTAAGTACATAACAGACAATATATATGCTATGTAAATGCATGTATTTTGCAATTTAATTATTGAAAGCAATTATTTAAATGATTACGTTCAATAGCAATGTTAACTCCTTAAGTTGGATGGTCCATTTCTGATTTTTTTTTACTTCAGTCATACAGATATTATTACAAATAATACAAACATCATATTTGTTTCATCTTTATTAACATTTAATTACAGTAGTATAAAATATAATAAAAATATTACTTAAATAATATGTAAATAAATATTACATAATCTAATAATTACTAGAGTAGTTTAATAAAATGGGATTCAAACAAAGTAAATTGGTTTGTGAATCCATAAAATCTTTTATTAAATTAACATTTTCCAAAGTTTTTGAACCATTTATAAAATGATGGTCTTTAAATAAGACGTTACATACAATAGATGTATTTTACATTTTAATATAATAATTTAATTAATAATAATTTAATTAATAATAATTTTAATATGAGCGTCCCTTGTAAATCTCCAGGCCTAAACCAGAGCTATTCACTAAATATGTTGAGATTAAACTTGAGGTCAACTAGAGGTGACATCTAGAGGTCAATATTAAAATTAAACATTCTCAACCCACATCTTAAAGCCCTGAACCTGCATGCAGATACTACAATACCTCATTTCAAACATTTAGTTTCCTATATGTACTGAGCTGGTCTGTTTCTCTTTTTTTCTTGTTTCTCTATGTCTCGTGGAG
Seq C2 exon
GAGGTAAATATGTGCCAAGAGCCATTCTAGTGGATCTGGAGCCTGGTACCATGGACTCTGTGCGTTCAGGGCCCTTTGGACAGATCTTCAGACCTGACAACTTTGTATTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000037997:ENSDART00000055380:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.135 A=NA C2=0.026
Domain overlap (PFAM):
C1:
PF0009120=Tubulin=FE(16.2=100)
A:
NA
C2:
PF0009120=Tubulin=FE(16.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTTGGGAGGTGATCAGCGATG
R:
ACAAAGTTGTCAGGTCTGAAGA
Band lengths:
214-1376
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]