BtaINT0165235 @ bosTau6
Intron Retention
Gene
ENSBTAG00000025124 | ZNF300
Description
zinc finger protein 300 [Source:HGNC Symbol;Acc:HGNC:13091]
Coordinates
chr7:64236526-64237033:-
Coord C1 exon
chr7:64236892-64237033
Coord A exon
chr7:64236649-64236891
Coord C2 exon
chr7:64236526-64236648
Length
243 bp
Sequences
Splice sites
5' ss Seq
TGGGTAAGA
5' ss Score
8.91
3' ss Seq
CTCTGATTTCTATTTAATAGGGC
3' ss Score
7.65
Exon sequences
Seq C1 exon
ATATTACTGTTACAGGGATTAGTATCTTTCAAGGATGTGGCTGTGGATTTCACCCAGGAGGAGTGGCAGCAACTAGACTCTGCTCAGAAGACCCTATACAGGGATGTGATGCTGGAGAACTATAGCCACCTAGTCTCAATGG
Seq A exon
GTAAGAGTGACTTCCCTATATAACTCAGACTTGACCAGTCAAGTACCTTTCCTTACCAGTCACTATAGTTAGGTGGTTCCTGTTGTGTATATAATGACTTTGATGATCTTAATACCTTCCCCAATGGAGGTCATTACTTTCTGAAGGTTAAATGAACTACTTCATTGTAGACCTCCTTAAACTGCATCTTCTCTTTTGCCTTCTGTGCAGTCTATCCCAATTCCTCTGATTTCTATTTAATAG
Seq C2 exon
GGCATCCAGTTTCCAAACCAGATGTCATTTCCAAGTTGGAGCAAGGAGAAGATCCATGGATCATTAAAAGAGACATACCAAATTGGATCTGTCCATATGAAGGTCAGGCAGATGGGCGACTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000025124:ENSBTAT00000035145:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.071
Domain overlap (PFAM):
C1:
PF0135222=KRAB=WD(100=85.4)
A:
NA
C2:
PF0135222=KRAB=PD(0.1=0.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm10)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGTTACAGGGATTAGTATCTTTCA
R:
CTAGTCGCCCATCTGCCTGA
Band lengths:
260-503
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]