DmeEX0005782 @ dm6
Exon Skipping
Gene
FBgn0020621 | Pkn
Description
The gene Protein kinase N is referred to in FlyBase by the symbol DmelPkn (CG2049, FBgn0020621). It is a protein_coding_gene from Dmel. It has 17 annotated transcripts and 17 polypeptides (14 unique). Gene sequence location is 2R:9252310..9285097. Its molecular function is described by: protein serine/threonine kinase activity; protein kinase activity; ATP binding; protein binding; GTP-Rho binding. It is involved in the biological process described with 9 unique terms, many of which group under: cellular component organization; regulation of wound healing; localization; cell division; post-embryonic animal organ development. 65 alleles are reported. The phenotypes of these alleles manifest in: cellular anatomical entity; array of sensillum rows; non-connected developing system; adult head; intracellular non-membrane-bounded organelle. The phenotypic classes of alleles include: female semi-sterile; some die during larval stage; increased mortality; phenotype. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in adult female stages.
Coordinates
chr2R:9267655-9284129:-
Coord C1 exon
chr2R:9283828-9284129
Coord A exon
chr2R:9268369-9268778
Coord C2 exon
chr2R:9267655-9267976
Length
410 bp
Sequences
Splice sites
3' ss Seq
GTATTTGTTTTGTGGGTCAGCCT
3' ss Score
3.45
5' ss Seq
GAAGTGAGT
5' ss Score
7.97
Exon sequences
Seq C1 exon
GAAATCAAATGAGAGCGCATTCGATAAAAAGAGATAAAGAAAATACTGTTCGGCATCAGGTGTTAAAGTGCTAAACAATTCCGAAAGCCAAAGGAAAGTAAACAAAAACGCGACGCGTGTGCTTGTGCTTGTGTGCGGCGCCAATAAAAGCCAAAAGCAAAATACTGACAACCAAAAAGATACACGCAACAACAAGAACCATATTCATAATAACCAACAACTAAATACTACAACAAGAAGCTACGGCATCGAGTGATTCAACGCGGCGCACCAAATAAACCATGTCGGATTCGTATTATCAG
Seq A exon
CCTGTTCCAGCTAATTACCGTGAACTTGTCAGCGCACACAAAACGGCTGTAAAATGACCAAAGTGAGGTAATAAACACGGCGAATTAACGAGAAACCCGAGTGAATAATGTCCAAACTGGTGAAGAGAATCCGCAGCATGATCAATCCAAGCAGCCAGCGGGATCGCGAAGATACCACCTCGGTATCCGCTTCAGAGGGCTTCATTGCTCGCAGGAAGTGCCACTCGCTGCCCCGAAGGAGCAATAGACACTCGACCGCCCGGCGATCAAACAGTGGTCTGTGGAATCGACTGGTCACCAATGTCTTCGGACCGGAAGATTCATATGACTTTGACAACGAAGGCGACAGTGGTGGTGCCGTATTTTACACCGATTCGGTGAACGGTTCAAACTATGAGATATCTGCAGAA
Seq C2 exon
GGCGAATACATCAAGCATCCCGTTCTGTACGAGCTCAGTCACAAATATGGTTTCACAGAGAATCTGCCGGAGAGCTGTATGTCCATACGGCTGGAGGAGATCAAGGAGGCCATTCGGCGAGAGATCCGCAAGGAGCTGAAGATCAAAGAGGGCGCCGAGAAGCTCCGCGAGGTGGCTAAAGATCGACGATCCCTCAGCGATGTGGCCGTTCTTGTCAAGAAGAGCAAAAGTAAACTTGCCGAGCTGAAGTCCGAGTTGCAGGAGCTCGAGAGTCAAATCCTCCTGACATCGGCCAACACCGCCGTCAATAGTAATGGACAAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0020621_MULTIEX2-3/3=C1-C2
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
In the CDS, with uncertain impact
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.286 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0218511=HR1=WD(100=62.0)
Main Inclusion Isoform:
FBpp0111810
Main Skipping Isoform:
FBpp0308399
Other Inclusion Isoforms:
FBpp0290726, FBpp0291760
Other Skipping Isoforms:
FBpp0087682, FBpp0087683, FBpp0300526, FBpp0308400, FBpp0308401, FBpp0308402
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGGCATCGAGTGATTCAACG
R:
GGCCGATGTCAGGAGGATTTG
Band lengths:
354-764
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)