DmeEX6009352 @ dm6
Exon Skipping
Gene
FBgn0027561 | CG18659
Description
This gene is referred to in FlyBase by the symbol DmelCG18659 (FBgn0027561). It is a protein_coding_gene from Dmel. It has 8 annotated transcripts and 8 polypeptides (6 unique). Gene sequence location is 2R:9123304..9132030. Its molecular function is described by: Rab GTPase binding; phosphatidylinositol phosphate binding; Rab guanyl-nucleotide exchange factor activity. It is involved in the biological process described with: regulation of Rab protein signal transduction; endocytic recycling; endocytosis. 8 alleles are reported. No phenotypic data is available. No phenotypic class data is available. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, at stages throughout the pupal period.
Coordinates
chr2R:9126181-9127877:-
Coord C1 exon
chr2R:9127419-9127877
Coord A exon
chr2R:9127027-9127162
Coord C2 exon
chr2R:9126181-9126213
Length
136 bp
Sequences
Splice sites
3' ss Seq
TGATTTAATCTATTCCACAGTAC
3' ss Score
6.23
5' ss Seq
AATGTTAGT
5' ss Score
-1.83
Exon sequences
Seq C1 exon
GTCACCAGTGATGAGCTGGGTGAAGTTGTGATTCTGAACTGTGATACGAAAATATTCGAAAGCCCATTCGACGATGTGCACAATCTGCCAACGGAGATTGTGTCACAGCTGAAGAAGCATCTGAATCACACACATGACCACATCGGCGACCGCATCTCGAAGATCTTCCTAAACGCACTGGTGCAGCTCATCGGTGGCTACCGGGACGCTGTGGAGTACCACGAGAACAGCAAGACGTTTAATTCCCAAAAATTCATTGAATCTCGGCCGGCGCACCTGCGCCCCTTTCTTGCCAAAATGATGGATCTACAGATCTTTGCGCAGTTCATTGACGATCGCCTAACGATGCTCAACAGCGGACTCGGCTTCTCGGACGAGTTCGAGCTGGAGACGGTGCGCTATGCGGAAAAGAAGAAGCGCGGTCGAAACTACGCCATCATGAAGAACCTTAAGGACAAG
Seq A exon
TACTTGGGAAAGCGCAGTTTGTCGCGATTGTTTGCCAAGAACAATACTTAGACTGCCCACCATGACAAATCCACCAAATCCAATTACCAATATATAAACGAGTAGAATGTTCATTCCCACACACCTTATTTGCAAT
Seq C2 exon
ACTAATCCGGCTGTAAAGTCTGCCGTCAAATCG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0027561-'11-10,'11-8,13-10=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.020 A=0.000 C2=0.727
Domain overlap (PFAM):
C1:
PF0214116=DENN=PD(8.6=10.5),PF0345514=dDENN=WD(100=43.1)
A:
NO
C2:
NO
Main Inclusion Isoform:
FBpp0297473
Main Skipping Isoform:
FBpp0087707
Other Inclusion Isoforms:
FBpp0308462
Other Skipping Isoforms:
FBpp0291660, FBpp0308463
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGCAAGACGTTTAATTCCCA
R:
GGCAGACTTTACAGCCGGATT
Band lengths:
257-393
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)