DmeEX6010533 @ dm6
Exon Skipping
Gene
FBgn0259221 | CG42321
Description
The gene ATPase 8A is referred to in FlyBase by the symbol DmelATP8A (CG42321, FBgn0259221). It is a protein_coding_gene from Dmel. It has 15 annotated transcripts and 15 polypeptides (12 unique). Gene sequence location is 2R:13414978..13439051. Its molecular function is described by: ATP binding; magnesium ion binding; ATPase-coupled intramembrane lipid transporter activity. It is involved in the biological process described with: phospholipid translocation. 25 alleles are reported. The phenotype of these alleles manifest in: larval multidendritic class IV neuron. The phenotypic classes of alleles include: viable; partially lethal - majority die; some die during pupal stage. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 12-24 hour embryonic stages, during late pupal stages, in adult male stages.
Coordinates
chr2R:13414978-13419085:-
Coord C1 exon
chr2R:13418987-13419085
Coord A exon
chr2R:13416575-13416606
Coord C2 exon
chr2R:13414978-13415615
Length
32 bp
Sequences
Splice sites
3' ss Seq
TAATCTGTCTGTGTGCGCAGTAC
3' ss Score
6.69
5' ss Seq
GGTGTAAGT
5' ss Score
8.07
Exon sequences
Seq C1 exon
CGTACATAATACCGTGTTTAAGACACTGACGGAGGCTGTTCGCGAAACGGAGATCCGACGCAGCGACATCTCCGAAGTGATGAACGAACCGCGATCTTC
Seq A exon
TACCATGTATGCCTTGGGATCGATAATTCGGT
Seq C2 exon
ATGGCTATGCGTTTTCCCAGGAGGAGGGCGGAGCTGTGCCGCAGTCTATCATTATACGTGCCTATGATACGAATCTGCCAAAGCCCGAGGGTAACTAAACCGAAAACGATGGGACTCTGGATCTCACGGTGCACATGTATATAGGAGAACGCAAGGAGCTACGATTTCGATTCACATTGGTATATATTTAGAGCACACTTCTGGTAGGCCAAACTAAAAAAACACACAAAACACATCACATTTATTATCTATGTGTCTTACACCAGCAGTAATTGAAAAACATTTCGTAGACGCCACTAAACATTTGAATATTGCGCTTATATGTAAGTTTATCTTTCTATTTGCTGTTATTTATTTTGTAAGATCGCGAGGAAAAAAAACACATTTAAGAGGCTAGAGTTCGGTTTTGTTTTTATTTTTTGTATCTGTCCCCTTGTCCGCGGCGCAGCTTTGTGATACGGATACAGACTTGCATTCTTTTTTAAAAAAGTCTATCTCTA
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0259221-'22-27,'22-25,25-27=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.029 A=0.000 C2=0.120
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Inclusion Isoform:
FBpp0297637
Main Skipping Isoform:
FBpp0289033
Other Inclusion Isoforms:
FBpp0289036
Other Skipping Isoforms:
FBpp0289032, FBpp0289039, FBpp0289040, FBpp0289041
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAGTGATGAACGAACCGCGA
R:
CGGGCTTTGGCAGATTCGTAT
Band lengths:
113-145
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)