DmeINT0019397 @ dm6
Intron Retention
Gene
FBgn0265998 | Doa
Description
The gene Darkener of apricot is referred to in FlyBase by the symbol DmelDoa (CG42320, FBgn0265998). It is a protein_coding_gene from Dmel. It has 16 annotated transcripts and 16 polypeptides (11 unique). Gene sequence location is 3R:28888073..28922772. Its molecular function is described by: kinase activity; ATP binding; protein kinase activity; protein serine/threonine kinase activity. It is involved in the biological process described with 14 unique terms, many of which group under: multicellular organism development; visual system development; negative regulation of biological process; positive regulation of immune system process; embryo development. 88 alleles are reported. The phenotypes of these alleles manifest in: intracellular organelle; cellular anatomical entity; interommatidial bristle; sex comb; extracellular matrix. The phenotypic classes of alleles include: phenotype; increased mortality; stress response defective; increased mortality during development.
Coordinates
chr3R:28921156-28921549:+
Coord C1 exon
chr3R:28921156-28921269
Coord A exon
chr3R:28921270-28921358
Coord C2 exon
chr3R:28921359-28921549
Length
89 bp
Sequences
Splice sites
5' ss Seq
ACGGTAATA
5' ss Score
6.25
3' ss Seq
AGCAATCATACTCTTTATAGCAA
3' ss Score
4.14
Exon sequences
Seq C1 exon
GTGCATCTTGTTCGAACTGTATCTGGGAATCACGCTCTTCCAAACGCACGACAATCGCGAACACTTGGCCATGATGGAGCGAATCTTGGGACAAATACCATATCGCATGGCACG
Seq A exon
GTAATACAATGCAGCTCATGCTTTTATTTCCAAAGGCGCAGTTCCCTTCACCGAAATCGTTTAACGTGTAGCAATCATACTCTTTATAG
Seq C2 exon
CAAAACCAAAACAAAGTACTTCTATCATGGTAAGTTAGATTGGGATGAGAAGTCCAGTGCGGGTCGCTACGTCCGTGATCACTGCAAGCCGTTGTTCCTGTGCCAGCTGAGCGACAGCGAGGACCACTGTGAGCTCTTCAGTCTGATCAAGAAGATGCTGGAGTACGAGCCGTCGTCACGCATCACGTTAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0265998:FBtr0336461:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=FE(12.1=100)
A:
NA
C2:
PF0006920=Pkinase=FE(20.3=100)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0289022
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0289020, FBpp0289023, FBpp0289026, FBpp0289027, FBpp0289028, FBpp0289030, FBpp0289031, FBpp0307567
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AATCACGCTCTTCCAAACGCA
R:
AACAACGGCTTGCAGTGATCA
Band lengths:
183-272
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)