DmeINT0020634 @ dm6
Intron Retention
Gene
FBgn0010225 | Gel
Description
The gene Gelsolin is referred to in FlyBase by the symbol DmelGel (CG1106, FBgn0010225). It is a protein_coding_gene from Dmel. It has 8 annotated transcripts and 8 polypeptides (3 unique). Gene sequence location is 3R:4235217..4241057. Its molecular function is described by: phosphatidylinositol-4,5-bisphosphate binding; protein binding; actin filament binding. It is involved in the biological process described with: barbed-end actin filament capping; actin filament polymerization; actin filament severing; actin polymerization or depolymerization. 7 alleles are reported. The phenotypes of these alleles manifest in: adult brain; mitochondrion. The phenotypic classes of alleles include: partially lethal - majority die; some die during pupal stage; viable; size defective; partially lethal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed at stages throughout the larval period, during early pupal stages.
Coordinates
chr3R:4236788-4237817:+
Coord C1 exon
chr3R:4236788-4236939
Coord A exon
chr3R:4236940-4237624
Coord C2 exon
chr3R:4237625-4237817
Length
685 bp
Sequences
Splice sites
5' ss Seq
AGAGTAAGT
5' ss Score
9.35
3' ss Seq
TTTTTGCTTGTCTCTTTCAGCCT
3' ss Score
9.95
Exon sequences
Seq C1 exon
ATGGCAGTCCTCTCCAGGTAATGCAAGCGAGCAGTTCGCTGTCCAGAAAGCTCTGGGGTGATAAGTACTCAGTTCCTGAATTTTAACAAAACTTTAGCAGTCGTACGCGACATCGCTAACACCAATTCGAGAAAACAGCCGTTTCAAATAGA
Seq A exon
GTAAGTGCGTAAAAGTGGCTGCGGTGTGATTCAACACCCGACTTTTGTGTTCATTTCTAGGTTTCGCCAGGTTTGCCCCATGTGGCAGTTGTGGTGTGCCTAGTTTGTGGCCTTTTATGGTCATGACTCACATATAAACAGTCGGATGTACTATTTAGGTGTATCTAGTTTAATTGCGCTGAGAGAGAGCACAATTTCATGTCTTGAACACCCTACAGAGCGGTGTATCAGTTGGAGTTGCGCGTAATTGTGAATAGAGTGCCTGAATGTTGTAAGCCATATCCACCTATTGTCCTTTTTCCCTGAAACCTGTATTATCCAAAAGAAAAGTCGCTTTCGTGTAGCAGACTGAAATCTTTCTGGTTCAAAAAATATAGTTTCGGTTGTGTTTACTAAAGAACTATCGAAGAAAACACCTGTTTAATATCAAATTTTGTGATACCCTACCTGCATTGCAAAGAAGACTTTTCGGAACGCATCATTTGGATAGAATAAAAACTGATAAACTATTTTGCGTCGGGCTGAGAAACGGACGTGGCTATAACAACTTGTTGGTGATACTAATCAAGAGAAAACCCTCATAGACTCGCAAGAGTCCTTTAGTGGAATATATATTTTTTTAGAGTAGTTCTGTTATCTTTGGTCAATTAAGCTGCCATAATAGCTTTTTGCTTGTCTCTTTCAG
Seq C2 exon
CCTGCTTGTGTTTTTGGCCCTCTCGTCCAGTCTGTGCTCAGCCGGCACCCTCAACGCCCGTCCTGCGTTTCCCGTACAATCCGGCGAAATTCAGCCCAGCGGCCAGAACAGCAAGCAGGCAGCCAGACGCGTGATGCATCCTTCCTTCGCCAACGCCGGCCGTACTCCCGGCCTGGAAATCTGGCGCATCGAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0010225:FBtr0078971:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
5' UTR
No structure available
Features
Disorder rate (Iupred):
C1=NA A=NA C2=0.307
Domain overlap (PFAM):
C1:
NA
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0078607
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0078608, FBpp0078610, FBpp0078612, FBpp0301195, FBpp0307688
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCAGTCCTCTCCAGGTAATGC
R:
CTCGATGCGCCAGATTTCCAG
Band lengths:
342-1027
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)