DmeINT0021642 @ dm6
Intron Retention
Gene
FBgn0001248 | Idh
Description
The gene Isocitrate dehydrogenase is referred to in FlyBase by the symbol DmelIdh (CG7176, FBgn0001248). It is a protein_coding_gene from Dmel. It has 10 annotated transcripts and 10 polypeptides (5 unique). Gene sequence location is 3L:8356404..8360906. Its molecular function is described by: magnesium ion binding; NAD binding; isocitrate dehydrogenase activity; isocitrate dehydrogenase (NADP+) activity. It is involved in the biological process described with: isocitrate metabolic process; oxidation-reduction process; NADP metabolic process; cellular response to oxidative stress. 38 alleles are reported. The phenotypes of these alleles manifest in: multi-tissue structure; organ system; cell; organism; multicellular structure. The phenotypic classes of alleles include: lethal - all die before end of pupal stage; fertile; phenotype; flight defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes.
Coordinates
chr3L:8356404-8360906:-
Coord C1 exon
chr3L:8360793-8360906
Coord A exon
chr3L:8357906-8360792
Coord C2 exon
chr3L:8356404-8357905
Length
2887 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAGT
5' ss Score
11
3' ss Seq
TGGTATGCTCTCCCGAACAGATG
3' ss Score
8.56
Exon sequences
Seq C1 exon
TCAGTTTCAGTTATTCGCTGCGTCTGATTGGGAGTGGTTCGAGATCTTTCGGTCTGCAATTTAATTAGTCTGTGCTCTAACATTCTAAATTCGGCATTTTAAATATTCTAAAAG
Seq A exon
GTAAGTGGAGCATTTGTTTAGCCTTATATAAACCGGTTCACACTCCACGCCAAACACCCACACTTCCGATCGCATTCCGCCCAGCTATTTACTGCACTCAAAGGCTGACTTGCAATTTATCTGAGCAAATAAAAAATGGTGGATTTCCAGATCGGTTGCAATACGTAAATTTGCCGCAATTCAAGCCTCCTTAATGCAAATAAGTTGCGCATTTTAACTTTATGCATAACCTCTGGCCCAACCTATTTGGGCCCGAACTTTCCCCACCGGTCTGTTTTCATATGCATTTTATTTGTCTGCCGCTGTTTTGTTTTTTTCTTTGCACCGGTTTGCCTCCTCGAAAAAGAGAGATACGGATAAAAAAAGTGTGAAGAGACGACAGTGTGAAAGAGGGGGATATATGTGGAGAGAGAGAGACACGTAACCCATTCGACAGGCCTTTTCACATCCACCTGATTCTCCAGGTGCTGTCCAAAGTTTAATCTTGAATTCGTGTCGACAGTTTTAATTAAACCTATACAATTACTATGCATATCTACAAATAGATGGTTCGAGAAAACCTCCAGATAGCTCGTATCTCATTGGGACTTTTGCATGCACAATTTGGAAATGTTTTTAACTTTCTAAGTTTGTCCTATGTTTTGAGTGCTTAAACAACTTAAACAGTCGACGTTTATTTTTTTCATTTATCAAACTAGAAGTCAGATCTGAATACAATTAATTACTATAATTAGATTATCGTAAACTTATATTTGATAAGGATAGATGGACTCTTTTATTAACTTGTCAGTCGAAAAATTAAAACCATAATTTTATTAGGTTTTCTTTTTTATTTCTAGCGAATTTTGTATTAACAGTTTCATAAAGTTCAAGGGTCAAAAGTCATCATGCAATCTTTTTATTTCAACAATAAATATTTCTGGGACTAGAAAAACACCAGATGAGAAAATATCAGATTAGATCTAGATAATTTATTCGCCCCGTCATTACCAGCTTATTTACCAATCGCAAGTCTTATCAATATCGTACTATGTTCGAATTCTCCCAGTTGGACTAGAAAAAAAAACGCTTGTGAAATTCGTTTGGTGAAAGCATAAATATTGTTATTATATATAATTGTGGGCGTGGCCTCAGCTTATGGCGTGTTCGGCGATCGCGCCACCTCAATATGTTAATTAGGTACACATGTACCTATAAAGACTTAGAGATATTTATATTAAAGTCAGACAATGGATCTATTAAATATGAGAAGAAAAATCCAAAAAAACATCACGCAATTGCTATTTTAATTTGGAAGTAAATGGCATTGACTGAGTAGATAAGAATGGTAGACGTTTCAAGATAATCAGAGCGAGCCTCCTTCGCAACAAAAAAAAAAAAAGATTGGCGCCCAAGTTCAAATATTATCTAAGCTTTAACATGTCAGTTCGACTATCGTTATCGATAGTACCGCACGTACCAGCTGATTTTGAATTCGGGTGAAGTTGAATAAATAAGCTTAATGCCACGCGCTTTTCTAATTTGTATTCCTTATCAAAACAAGTCGGCCGTGAACTTTAAATAAAAGTGGCTAGCCCGCAATAGTGGAAAAGTTTTTCGCCAGCTGATAAACGCAACAGTTGTCATTCGGAAGAGAGGGACAATGCCACCCGCAATGCGAAAGCGATTGACAAGTCAGCCGGCGATAACGATTACATGTTTACGCGTTCGCCGAGTGCTTATTGTTTTTTATACTCTCGTTACATTATTAATTAAAACACTTGCTTTTAAGTGTGCCGAGTGTTGTGAGAGCAGCTTATCAAAGTCCGAGGTTCTAAATCTTATCTGACTGGGCATTTAGAACGTTGTGATTACGCCGATTGCGCAACAATTTGACGATTGTACGCCACCCAAGAGAGTCGGAAACTGTAGCGCTTAACGGCACTTTACAGTTATCAGTTACGGTTAGAGCGCGTCTGACGCCCACAGCATTTAATTTTGCTTTGTCCGAAAATTGTTCTCTTTCCCGGGACTCCAGTGTCATATTGTCTCGTATTGTTTCACATTGGTGACAAACACTATCGTCTCTCGTTTCCAGTCGTCGGTCGGTTCATTCATAATAATAATACCCACATCCACACCAATCCGGTTGTAAAATGTTCGCCCTGCGCCGCACAGCCGCAATGATGACGTCGGTCCACCGGCAGCCGCATCTCAGCCAGGCCGTTTTTCGGGCCAATTTCGCCATCAGCGCCGCGGTAGGTCCTCCTTGTATCCTCGCCCTCTGTCGCTCTAAATCTCTTTGCTTACTAAATTTCGGAATAGGGTGGGTCGATGAGCAGGAGAAAAAGATATTTTGAATTTTTTGAAGTCGGTCTCAATATTAAGATTATAAACAATCACGTAGTCACCTGTGCTGCTAGCATCCGATAAATAGGATTACTATATTTACTGTTTATACAAAAAAATTGAACAATTTTTTCTTAAATTTATAACCTATGAATGCAAAGTAGAGATATTAACTTTTAGGCAAGTACCTTAATAATTACGTCGTCACGACGAAATATTAAATATTACAAAGTAAAGATAAGGGATTACTTTTGAAACACGGTGTATTTTAATTTCCATCCATCAATATCGACCCACCCTATCCCAGGCTGATCAAAGTTTACTTTGCTTTGACCAGTTGTCAGTTTATTATCGTCATACATATAAGTTTCGCCTGTATCGCCTGTGCTTAGGACCCCAAAACCCATAAGATATCCATGGATAATGAAAGGATGTCCGCCGTGAGCGAGGTGAATACCCCAATACCGCAGCATTCAGACACAAATCCATTTTTCATGTAGCTAATAAGATTCCCATCCTGCCCTTCAAGCACTCAAGTGTGGTATGCTCTCCCGAACAG
Seq C2 exon
ATGGCCCAGAAGATCAAAGCCGGTCCCGTTGTGGATGTCCTCGGTGACGAGATGACCCGCATCATTTGGGACTCCATTAAGAGCCAACTGATTCTGCCCTTCCTGGACATTGAGCTGCATACCTACGATCTTGGTATTGAGAACCGCGATAAAACCGAGGACCAGGTCACCATTGACTGTGCTGAGGCCATCAAGAAGTACAACGTGGGCATCAAGTGCGCCACAATCACTCCCGACGAGAAGCGCGTAGAGGAGTTCAACCTGAAGAAGATGTGGAAGTCGCCCAACGGTACCATCCGTAACATCTTGGGAGGAACCGTCTTCCGTGAGGCAATCATCTGCAAGAACGTGCCCCGTCTGGTGACTGGCTGGCAGAAGCCTATTGTGATCGGTCGCCATGCCCACGCCGATCAGTACAAGGCCGTCGACTACGTGGTTCCCGGACCCGGCAAGCTGACCCTCACCTGGAAGGGAACCGACGGTCAGGTGATCGATGAGGTCATTAATGACTTCAAGGGCCCCGGTATTGCCCTGGGAATGTTCAACACGGACGATTCCATTGTGGACTTTGCCCACGCCTCTTTCAAATATGCTCTGGACCGCAAGCTGCCGCTGTACATGAGCACCAAGAACACCATTCTGAAGAAGTACGATGGACGCTTCAAGGACATATTCGAGGACCTGTACAACAAGCAGTACAAGAAGGAGTACGAGGCGGCGGGCATCTGGTATGAACACCGTCTCATCGACGATATGGTTGCTTATGCCATGAAGTCGGAGGGTGGCTTCGTGTGGGCCTGCAAGAACTACGACGGTGATGTGCAGTCGGACTCCGTTGCCCAGGGCTACGGATCTCTGGGTCTGATGACCTCCGTGCTGCTATGCCCCGATGGCAAGACCGTGGAGGCTGAAGCCGCTCACGGAACGGTGACGCGTCACTTCCGCTTCTACCAGCAGGGCAAGGAGACCTCCACCAACCCCATTGCCTCGATATTCGCCTGGACCCGTGGCCTGCTGCATCGTGCCAAGCTGGACAACAATGAGCCACTGAAGCAGTTCGCCGACACCCTTGAGCAGGTGTGCATCGACACAATTGAGAGCGGCGCCATGACCAAGGATCTGGCCATCTGCATTAAAGGCAACATCAACGCCGTTACTCGCAGGGACTACCAGGAGACTTTTGAGTTCATTAACACGCTGGCCAAGAACCTGGAGGGTGCCCTGGCCAAGAATGCCGTTGCTGCCAAATGACTATCCGGCACTCAGTCCGAGCAGCAGGCCTCGCATCTCTAGAAAACTACTCAGCGATGCCTTGTCCTCGTCACCATATTCACAATATTTCCATTCGTTTTTGTAGATGTGCATGTCCTAGGCTAAAAATCCGTGTCCTCGATGCATGTTTCCCACTGTTTCATTGTCTCTGCGAGACGCAAAAAAAAACCCTGATTTCCCTTTAACGTCTAACGGAAAAGAAATTTCAAATAAATGTATTTTTATACCGG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0001248:FBtr0076667:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
5' UTR
No structure available
Features
Disorder rate (Iupred):
C1=NA A=NA C2=0.001
Domain overlap (PFAM):
C1:
NA
A:
NA
C2:
PF0018015=Iso_dh=WD(100=94.0)
Main Inclusion Isoform:
FBpp0305272
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0076391, FBpp0076394
Other Skipping Isoforms:
FBpp0076390
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)