DreEX0013146 @ danRer10
Exon Skipping
Gene
ENSDARG00000077582 | ank3b
Description
ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2]
Coordinates
chr12:7620912-7631399:-
Coord C1 exon
chr12:7631318-7631399
Coord A exon
chr12:7624452-7630244
Coord C2 exon
chr12:7620912-7620986
Length
5793 bp
Sequences
Splice sites
3' ss Seq
TTTCATTTGTCTGTTTAAAGAAA
3' ss Score
6.39
5' ss Seq
TTGGTGAGC
5' ss Score
7.43
Exon sequences
Seq C1 exon
ACTGAAAAGCCAGAACGACGTCATACCTTTGCATCTCTAGCCTTACGTAAGCGCTACAGCTATCTGACCGAGCCTGGAACGA
Seq A exon
AAACAGTTGAACGAAGTACAACAAGAACACTGCCTGCTGGTTACACTCACAAGCCTGTCTTTTCAACAAGATCTTACCAGGCATGGTCGACTGCTCCTATTACCGTCCCTGGGCAAACAAAATGTGGACTGGGTTCCCTCTCCAGTTCACCTTCCAACACGCCATCTGCTTCGCCACTAAAGTCTGTATGGTCCATTTCCTCTGCCTCTCCAGTCAAGTCCACCCTAGGAACATCAAATGCTTCTCCTGCAAAATCAATTAGCGACGTTGCATCACCCATTAGAACATACAGATCAATGTCATCACCCATAAAGACTGTGGTTCAACAACCCCAAAATCAAGTTCCAATATCTTCCAGTCTCCTATCTTCGCCAGGTAAAACTGGCACAGATCCTGTGTCTATTAAGGGGCTTGCTGCCTCATTGTCCTCAAGAGCTAACCTGATTTCGTCACCGGGATCCATGCTAGACAGAACTTTCACAACAGTGACCCAATCTGACTCTATAAAGTCAACCACGAATACCTACACATCATTCAAATCCACCCTGGCTCCCACAAGTATAGCAGTGTCCTCTCCCATTCGAAATATATCAAGCCTATCCTCTATTAAATCCACCACAGATGCAGCCAGGGGGACATTTTTGTCATCACTTTCTTCATCAGCTAAGCCTACAACAGCCAGTACTGAAACAGTCATGTTAAATGGATCCGTTTCACCATTAAAATATCCCATATCCTCCTCTTCATCTTCCCGTCTGTTTTCAGGAGGGGCTTATAGCTTACAAGAACGGATACAGGCCACAACACAAGCAGCGACAGCCAGTGTTGGTGCAGCCTTCAGTGAGGCTGAAAGAACAGTTACTGGTGGCTCGAAGTCAAACTACAGTACACTAAAATCTGTATCGTCACCTTTAAGATCACACTTAACAAGTTCTGTTTATGAGTCCCTTCGATCACCAGTCACCACTACCACGCCAATGACATCTGTTTCCATGTCTGTTCCAGTATTTTCTGTGGTCAGTGTGCTTCCTGAAACACAGGGGAGGAAACTTCCAGAAAAAATAACAATCACACCTCAGACACACAACCAAACACAGTCCTCTACAACACGAAAGAAACCTGCAAAGCCCTCACTTTTCATTTCTCCAACAGTCCTCAAAGCAGCAGCCACACCCACATTGACTTCCAATCAAGAGATTCTTAAGGATGTAGCAGATATGAAGGAAGATCTGATGAGAATGACAGCAATCTTACAAACTGACTCTTCAACTTCACCCAAAAGTTTCCATTCAAAGGTTTCTAAAGAACCCAAGATAGAAGATGAAGAAACTTTCAGTTTGGTCGAGAAAGTTAAAGAGGACCTTGTCAAAGTTAGTGAAATTCTTAAGAAAGATGTTCTGAGTGAGGCCAAAAACTCTCGAAAAGAGAGGGCATCAGAAGATGAATGGGAAGAGTTCTCTAAAGATGAAATTGAGGAGGCACGACACAGTGCCCTGCGATCCCTGCCAACATTCGAATCAACACTTCCAGTTGGGCCACAGTCAGTGCCAGACAAAGATCTCAATTTAGCCAAAGTGGTTGATTACTTAATGAATGATCTTGGTGCTAGTTCCCTTTCAAAAATAGCTGATGTAAAGAGCAGATATGATGAAATAAAGAAAGAAGGTGAGGAAAAACAGAAGCGCACCATGAAGCCTGAGCACAAGCTCAAAATGCCACCCACCGGTATGAGGACCTCACCTTCAGAGAAAGACTTAAGTAAATTAACTCAAGAAACATATAGTGGCACTGACGCTATCTTGGAATCTCCTGATGACTTTTCCCATGAGCAAGATAAAAGTCCTCTCTCAGATAGTGGTTTTGAAACCAGAAGTGAAAAGACCCCATCGGCTCCTCAAAGTGCAGAGAGCACAGGGCCCAAACCCCCATTCACAGATGTGCCTATTCCCCCTGTCATAACAGAAACCAGAACTGAGGTTGTTCATGTCATCAGGAGCTATGAACAACCTGATGAGGTCTGTGAACCATTAATGGATGAGGCAGCAGCTTTGAAGCCTCCAGTTGAACCTGAGTCAGCCGTTAGCTCAATCAATGATAAGGTAAAAGCCTTGCAGATGAAAGTCAGCTCTGACGAGTTGGCATGCCTCAAAGAAGAAACTCATATTACCACTACAACTCGAATGGTCTACCACAAACCTCAACTGAAAGATGCTGGTTCTGACCGGATTGAAGAAACAATGTCAGTCCGAGACATAATGAAAGCTTTTCAGTCTGGAAGAGACCCTTCCAAAGAATTAGCTGGTTTATTTGAGCACAAAGCTGGCCAAGATACCATTAAAGGAGATGAATTGTCCCCAAGGTTCCTTGAAAAGGACAAAAAACCCAAAGTTGAGAGAATTATTGAGGTGCACATAGAAAAGGGCAATACAAACGACCCGACTGAAGTTATAATCAGAGAAACTAAAAAGCATCCAGAGTTTATATGCAAGAGTGACCGAGGTCTCAGGGAGCTTGTTGAGAGGGTTGATGGGGATTATGAGGTTCTTCAAGATGAAGAGGAACTTACTGCAGAGGAAACACTGCCGTCCTTCCTAGACACGTCCAGAGTTAATACACCTGTCTCTCAAGAGGAAGAGAGTCGTCCCAGTTCAGCTCAGCTTATTACTGATGAATCATATAAAACGTTAAAGCTTCTAAGCCAGCATTCAATTGAGTATCATGATGACGAGTTGTCTGAGTTGAGAGGGGAGTCATACAGGTTTGCTGAGAAAATGTTGCTCTCAGAAAAATTAGACATATGCCATTCTGATACTGAGGATTCAGTGGCAGACCAGACACATAGCATAGCCACTGAAGTACAAGGGGCAGAAGGCGTATATGGAGAAAAAGTAACTGGCCCAAGGAAGGAGTCCAAATTAAGGTCAGCAAGAGATGCATCAGAGAAATCAGGTAAATATCCCCCACAAGAAGAGCAATTTGACAAAGTTACAGTATTACATTACACCACAGAACCAGGAAGCCCTAAACATGCCGTCTGGATGCGATTTACCCAAGATAGGCAAGACAGGAATAGAGAGAAACTTATTTATGAGGACAGAGTTGATCGGACTATTAAAGAGGCAGAGGAAAAACTGAGTGAGGTCTCACAGTTTTTTAGAGATAAAACTGAAAAGCTAAATGATGAGCTTCAGTCTCCCGAGAAAAAGAAACCAACAAGACTTTCACGGGAGACACTGTCAGGGCCGAGCTCAATGTGCAGCAGCCCTGAGAAAAAGCAAAAGAGCACAGCTGATGAATGGGAGAAAGGCAGGCAGAAAATGTTTGGGAGTACAGCTGAAAGGAAAAGTGCGAGCCTCCCAAACAGTCCCGAGAGGCATAAGCTGTCACAGTTCAGTGAGGATAAGCCAAAACAGCATGAATCTCCTCAGCAGGCAAAGACAGGTAATACAGGCCCCCCAGTCCCAGTTAAGACATCTAAAGTGAGTTCTTTTAGGCAGAAGTTTGAAACAGAAGCACAGAAACAGGATAAGAATATACCTATAGCACAATCCAAAAAATCACCAGAAAGTAAGTTACCAGTTTATCAATTTTTCACTGGTTCAAAACCGTCAAAGTCAGACTCTGCAGATGTAGGACAGTCACCTTCTAGAAAACTAACAGAAATCGATAAAAGTAAAGTAGACCCTCAGAGTTGTAAATCTCAAGGTCCTCAAAGAACTACTGTTTCAAAGCAATCCGGTGAAAAAGCTTTACTTGAAAAATCGACTACATCAAATAATGTTGCAAAAAAAGCCTCGCTGTTTGAGAAGATTAAGGATCAGGGGCTTGATACAACTCTAGAGAGCTCTACTAAAGTGCAAGACTTGAAAGATGGATATAGTGCAGACAGACATAGTGCAGTTAATGATATTAAAGAACAACGGTACCTAAAAAGTGACATTAAAAATGACTCCTTTAAAACAGATGCCTTATCGAAGGATAATGTCAGTAGAAACGACAGCCAGATAAAGACCCTTAAGAAAAAGACAGAATCCCATATTCCCATGAGAACAACCTCTAGTTCCTTAGACAATAACCAGTCAAAGAAGATGAATCAAGATACAGTCAGTAAATCATCTGAAAGGAGCCCATCGCACATACCTACCCTGACAAGACCAAGGGTCCAGGGCCGTTTAGAGTTACAGAAAGAAGATGCTTCAACAACGAAACCGGAAGAAGTCTCTGTTGAAAGTAAAACAAGTGACACTAGTTCAGTGGAAAAATTCACAGTTACAACAAGAGAAAGTTTCAAAGGGCTTAAAACTTTACCAGTTTATGTAAGTGTGCAAATGGGTAAGCAGTCTGATAAAGACATGGGAGGAGGAACAAATGGAACAATAAAAAAGGTATCTGGTTCAGAAAGTCGGACAGTTTATGCAGTTAAACAGAAACAGCCCACATCTCCTCAAGGCAGCCCAGATGATGACACCTTAGAACAGTTCTCTTTTATCGATAGCTCTGGCAAAAGTCCTATGACCCCAGAGACCCCAAGCTCAGAAGAGGTCAGCTATGATCTCACTTCGAGAACTCCTGATCCATTTATTGCATTTATGCCAGGTATTACAAGCCCGATAGCTGAGGTTTCAGAAGAATCTGAAGATAGTGAACAAGGTAAAGATCATTCTTTGAAAAAATCTGGACCAGAAGTTCAGGAAACAAAAGACAAAAGAGTTGCATATATAGAGTTTCCTCCTCCACCACCCTTAGATTCTGATTCATCACAGCCTGAGAAAAAAGGGTCGACTCCTTCCTCAGAAGCTGAGACTGAAATGATGGAAGTAAATCTCCAAGAGGAGCATGATAAACATCTTCTTGCCGAACCCATAATAAGAGTCCAACCTCCATCCCCTGTGCCCCCAGGAGCTAATGATAGTGATTCAAGTGACGAGGAATCAGTCTTTAAACCCATTCCAATAAAGAAATATACCTTTAAAATTGATGAGAACCAGGACTTGACAGACAGCCAATCAAAAAAACAAGACAAGAATGGTAATGATAAAGAACCAGGGGAAAATGGTAATGGAAAAACAGAAGATTATGATTATGAACAAAATGGCAATGATCAGTCCATCACAGACTGTTCAATAGCAACAACAGCAGAGTTCTCTCATGATACAGATGCCACTGAGATTGACTCACTGGATGGATATGAACTTCAGGATGAGGATGATGGCTTAAGTGAACCTATAGCCAAACCTTTTGGATTTCCCAATGACAACAGAAAAGACATTTGGGCATCAGATAATATGTCTAGACCAAGTGACCGTTGCCAAAGTAAACTTGAAGTAATTCAAGAAGAATCACCAGCTGAGGACTGCAAAAAGACCAAAGCTAAGATTGATCCTTCAAACAGAACGAATGGAAAAGACGAAAAGGATGGAAAACAATCTGATCAAGGACTTTCGGACACTTATTTTAGTTACAAGTTAGATGAGGAGTTTAATACTCCTTTTAAAACTGTTGCAACAAAAGGCTTTGATCCTTGGTCAAGCAAGGGAGGAGAAGATGAAGTAGTGGATGCTAGAATTAAAGACGATGAACCCAAGCCATTTGGGTTAGCAGTAGATGACAAGTCTGCTGCAACAACCCCTGACACAACTCCTGCAAGAACCCCAACTGATGAAAGCACACCAACTAGCGAGCCTAACCCTTTCCCCTTCCACGAAGGGAAGATGTTTGAGATGACCCGCAGTGGTGCGATTGACATGAGCAAGAGGGATTTTGTTGAAGAGAGGCTGCAATTTTTTCAGATTG
Seq C2 exon
GCCCTCAGACCCCCTGTGAAAGAACAGATTTGCGAATGGCTATTGTAGCAGATCATCTCGGCCTGAGTTGGACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000077582_MULTIEX1-3/3=2-C2
Average complexity
C1*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.362 A=0.749 C2=0.247
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0053117=Death=PU(18.1=57.7)
Main Inclusion Isoform:
ENSDART00000088100fB1186
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 1 annotated functions for this event
PMID: 25552556
Proximal axonal polarity as well as assembly of the AIS and normal morphogenesis of nodes of Ranvier all require a heretofore uncharacterized alternatively spliced giant exon of ankyrin-G (AnkG). This exon has sequence similarity to I-connectin/Titin and was acquired after the first round of whole-genome duplication by the ancestral ANK2/ANK3 gene in early vertebrates before development of myelin. Non-specific knockdown of Ank3 completely abolishes AIS clustering of AnkG, B4 spectrin, VGSC, and NF186. Rescue with 480-kDA isoform (with giant exon; MmuEX0004933) completely restores this phenotype, whereas rescue with the 270-kDa isoform (with a massive ALTD in the giant exon) partially restores the phenotype. Rescue with 190-kDa isoforms fails to restores any part of the phenotype. Specific knockdown of the 480-kDa isoform completely abolishes AnkG staining at the AIS as well as clustering of its associated binding partners, _4 spectrin, VGSC, and NF186.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]