DreEX0021688 @ danRer10
Exon Skipping
Gene
ENSDARG00000009021 | chrna1
Description
cholinergic receptor, nicotinic, alpha 1 (muscle) [Source:ZFIN;Acc:ZDB-GENE-980526-137]
Coordinates
chr6:10587730-10593496:-
Coord C1 exon
chr6:10593304-10593496
Coord A exon
chr6:10592075-10592217
Coord C2 exon
chr6:10587730-10587839
Length
143 bp
Sequences
Splice sites
3' ss Seq
CTAATGTTCATGTATTGCAGGAC
3' ss Score
8.5
5' ss Seq
GTGGTGAAT
5' ss Score
1.79
Exon sequences
Seq C1 exon
GACTGCCACACGAGGCACACGAGTTCATCTCTCTCTCTCACTCATATTGACAGAGTTCCAGCAGAAGTCAGGACGCCAATCATGCTCGTATTGACTATCTAATCGTTCAGTCAGCTATAAGGACTTTTCCCAGTCATATCAACAGAAATGAATTACTTCATATTGATTTTGCCTATTTTGCCTTACCTCTACG
Seq A exon
GACCAGCAGTCTGCTCAGAAGATGAGACCAGATTAGTAAAGACTCTGTTTACCGGATATAACAAGGTTGTTCGTCCTGTTAGCCATTTTAAGGATCCAGTGGTAGTTACTGTGGGGCTGCAACTCATTCAGCTGATCAGCGTG
Seq C2 exon
CAATGGAAGGATGTGCATCTGCAGTGGAATCCGGACGATTACGGAGGCATCAGGAAGATCAGGATCCCCTCGACCGACCTCTGGAAACCCGATTTAGTGCTTTATAACAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000009021_MULTIEX1-1/2=C1-C2
Average complexity
C3*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0293118=Neur_chan_LBD=PU(29.5=80.9)
C2:
PF0293118=Neur_chan_LBD=FE(17.3=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGCACACGAGTTCATCTCTCT
R:
TGATCTTCCTGATGCCTCCGT
Band lengths:
242-385
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]