DreEX0021713 @ danRer10
Exon Skipping
Gene
ENSDARG00000052764 | chrnb3a
Description
cholinergic receptor, nicotinic, beta polypeptide 3a [Source:ZFIN;Acc:ZDB-GENE-040108-1]
Coordinates
chr1:21702857-21721303:-
Coord C1 exon
chr1:21721001-21721303
Coord A exon
chr1:21710965-21711116
Coord C2 exon
chr1:21702857-21702901
Length
152 bp
Sequences
Splice sites
3' ss Seq
GTTCCACCATCTCTTTCTAGCTC
3' ss Score
8.47
5' ss Seq
GTGGTAAGA
5' ss Score
8.24
Exon sequences
Seq C1 exon
CTCTTGTGCTCGCATCGGATGGAGCAGGATGAAAACCCCACTCCGTTGATTATTAATTTACAGCTTTGTCACACAGCGGGTCGCAGTACATCTGATCGTTTTTCTAAACGGCCCGTGTCCAGCCGTATGAATAACGGACACTATATATTTACTCCCCCCCTTTTGTCGATGTCAGGGACTCACTATGCAAACAGCATGAAATAATTCCATCGCACGAAGGACCTGGAGCCGGGAAAGACAACATGAAGTTACAAATCTCCGGTATTCTGCTCGTAGCGGCAGTAGCCTATGCAACAATAGAGG
Seq A exon
CTCCTGAGGAGTTTGTCTCATTGGCTGAAATGGAGGACACTTTATTGAGGAATCTTTTTCGGGGATATCAGAAGTGGGTGAGACCTGTCCTTCACGCCAACGACACGATTACTGTTCGCTTCGGTCTGAAGATCTCACAGTTGGTGGATGTG
Seq C2 exon
GATGAAAAAAATCAACTGATGACCACAAATGTCTGGTTGTGGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000052764-'0-1,'0-0,4-1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0293118=Neur_chan_LBD=PU(19.0=76.5)
C2:
PF0293118=Neur_chan_LBD=FE(6.8=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTTTCTAAACGGCCCGTGTCC
R:
CTCCCACAACCAGACATTTGT
Band lengths:
249-401
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]