DreEX0048717 @ danRer10
Exon Skipping
Gene
ENSDARG00000062003 | myo5b
Description
myosin VB [Source:ZFIN;Acc:ZDB-GENE-031219-7]
Coordinates
chr21:22423465-22429728:-
Coord C1 exon
chr21:22429646-22429728
Coord A exon
chr21:22426545-22426622
Coord C2 exon
chr21:22423465-22423663
Length
78 bp
Sequences
Splice sites
3' ss Seq
CTCTTGCACTCGGATGGCAGTAA
3' ss Score
1.99
5' ss Seq
CAGGTTGTG
5' ss Score
-0.3
Exon sequences
Seq C1 exon
AATTCCAACGATGTGTCTGAGTGGACCGACAACAAGAGCCCTGTGAACACGGAGGACCGCGTGGATGCGCCTGCATCAAAGAA
Seq A exon
TAAAGCTCAAGACTGGGGTTATTTGAATGAAGATGGCGAGCTAGGCCTGGCCTACCAGGGTCTAAAGCAGGTTGCCAG
Seq C2 exon
ACTGCTGGAGACCCAACTGCAGACCCAGACTCGACAGCACAGGGATGAGTTGGAAGCCCTTCACACACAGATCGAGCTCCTGAAGGACAATCTGGAGAAGAAGCAGGACATGCTGGACCACTTCTCCACACTTTCTCCTGATGCTCTGGTGGAGTTCAGCGTGCAACAGGAGATCACACGCCTCACCAATGAAAACCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062003_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=0.257 C2=0.272
Domain overlap (PFAM):
C1:
PF102794=Latarcin=FE(16.4=100),PF131661=AAA_13=PU(17.2=92.9)
A:
NO
C2:
PF102794=Latarcin=PD(25.5=62.7),PF131661=AAA_13=FE(43.7=100)
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACAAGAGCCCTGTGAACACG
R:
GAAGTGGTCCAGCATGTCCTG
Band lengths:
177-255
Functional annotations
There are 1 annotated functions for this event
PMID: 19008234
Rab10 can interact with myosin Va, myosin Vb, and myosin Vc. Rab10 localized to a system of tubules and vesicles that have partially overlapping localization with Rab8a. Both Rab8a and Rab10 were mislocalized by the expression of dominant-negative myosin V tails. Interaction with Rab10 was dependent on the presence of the alternatively spliced exon D in myosin Va (HsaEX0041195) and myosin Vb (HsaEX0041208) and the homologous region in myosin Vc. Yeast two-hybrid assays and fluorescence resonance energy transfer studies confirmed that Rab10 binding to myosin V tails in vivo required the alternatively spliced exon D. In contrast to these previous work, the authors found that Rab11a can interact with both myosin Va and myosin Vb tails independent of their splice isoform.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]