MmuEX0030537 @ mm10
Exon Skipping
Gene
ENSMUSG00000025885 | Myo5b
Description
myosin VB [Source:MGI Symbol;Acc:MGI:106598]
Coordinates
chr18:74723757-74731843:+
Coord C1 exon
chr18:74723757-74723857
Coord A exon
chr18:74728871-74728948
Coord C2 exon
chr18:74731645-74731843
Length
78 bp
Sequences
Splice sites
3' ss Seq
TCTCTGTACTTCATTTCTAGCCA
3' ss Score
7.86
5' ss Seq
CAGGTCAAA
5' ss Score
2.44
Exon sequences
Seq C1 exon
GAGCCGAACATCAATGCCAGAACAAGTTGGCCCAACAGTGAGAAGCACGTGGACCAGGAAGACGCCATTGAGGCCTATCACGGGGTCTGCCAGACAAACAG
Seq A exon
CCAGACTGAGGATTGGGGATATTTGAATGAAGATGGAGAACTCGGCTTGGCTTACCAAGGCCTAAAGCAAGTTGCCAG
Seq C2 exon
GTTGCTGGAGGCCCAGCTGCAGGCCCAGAGCCTGGAGCATGAGGAGGAGGTGGAACATCTCAAGGCCCAGGTGGAAGCCCTGAAAGAGGAGATGGACAAACAGCAGCAGACCTTCTGCCAGACCCTGCTGCTCTCCCCAGAGGCCCAGGTAGAATTTGGTGTCCAGCAGGAGATATCCCGGCTGACCAATGAGAACCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025885_CASSETTE4
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.775 A=0.074 C2=0.239
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCCGAACATCAATGCCAGA
R:
CTGGGCCTTGAGATGTTCCAC
Band lengths:
171-249
Functional annotations
There are 1 annotated functions for this event
PMID: 19008234
Rab10 can interact with myosin Va, myosin Vb, and myosin Vc. Rab10 localized to a system of tubules and vesicles that have partially overlapping localization with Rab8a. Both Rab8a and Rab10 were mislocalized by the expression of dominant-negative myosin V tails. Interaction with Rab10 was dependent on the presence of the alternatively spliced exon D in myosin Va (HsaEX0041195) and myosin Vb (HsaEX0041208) and the homologous region in myosin Vc. Yeast two-hybrid assays and fluorescence resonance energy transfer studies confirmed that Rab10 binding to myosin V tails in vivo required the alternatively spliced exon D. In contrast to these previous work, the authors found that Rab11a can interact with both myosin Va and myosin Vb tails independent of their splice isoform.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types