RnoEX0057419 @ rn6
Exon Skipping
Gene
ENSRNOG00000014104 | Myo5b
Description
myosin Vb [Source:RGD Symbol;Acc:621347]
Coordinates
chr18:70695462-70703462:+
Coord C1 exon
chr18:70695462-70695562
Coord A exon
chr18:70700523-70700600
Coord C2 exon
chr18:70703264-70703462
Length
78 bp
Sequences
Splice sites
3' ss Seq
TCTCTGTACTTTATTTCTAGCCA
3' ss Score
7.35
5' ss Seq
CAGGTCAAA
5' ss Score
2.44
Exon sequences
Seq C1 exon
GAGCCGAACATCAATGCCAGAACAAGTTGGCCCAACAGCGAGAAGCACGTGGACCAGGAAGATGCCATTGAGGCCTATCACGGGGTCTGCCAGACAAACAG
Seq A exon
CCAGACTGAGGATTGGGGATATTTGAATGAAGATGGAGAACTCGGCTTGGCTTACCAAGGCCTAAAGCAAGTCGCCAG
Seq C2 exon
GTTGCTGGAGGCCCAGCTGCAGGCCCAGAACCTGAAGCATGAGGAGGAGGTGGAGCATCTCAAGGCCCAGGTGGAGGCCATGAAGGAGGAGATGGACAAACAGCAGCAGACCTTCTGCCAGACTCTGCTGCTCTCCCCAGAGGCCCAGGTAGAATTTGGTGTCCAGCAGGAGATATCCCGGCTGACCAATGAGAACCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000014104_CASSETTE4
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.794 A=0.074 C2=0.239
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAGAACAAGTTGGCCCAACA
R:
ATCTCCTCCTTCATGGCCTCC
Band lengths:
178-256
Functional annotations
There are 1 annotated functions for this event
PMID: 19008234
Rab10 can interact with myosin Va, myosin Vb, and myosin Vc. Rab10 localized to a system of tubules and vesicles that have partially overlapping localization with Rab8a. Both Rab8a and Rab10 were mislocalized by the expression of dominant-negative myosin V tails. Interaction with Rab10 was dependent on the presence of the alternatively spliced exon D in myosin Va (HsaEX0041195) and myosin Vb (HsaEX0041208) and the homologous region in myosin Vc. Yeast two-hybrid assays and fluorescence resonance energy transfer studies confirmed that Rab10 binding to myosin V tails in vivo required the alternatively spliced exon D. In contrast to these previous work, the authors found that Rab11a can interact with both myosin Va and myosin Vb tails independent of their splice isoform.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]