DreEX0050049 @ danRer10
Exon Skipping
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23028473-23031977:-
Coord C1 exon
chr9:23031870-23031977
Coord A exon
chr9:23031672-23031779
Coord C2 exon
chr9:23028473-23028577
Length
108 bp
Sequences
Splice sites
3' ss Seq
CTTTTCTTTATTCCATTCAGAAC
3' ss Score
9.27
5' ss Seq
AATGTATGA
5' ss Score
2.55
Exon sequences
Seq C1 exon
CTCAACTACCGTCATGACTATGCCACAAACGTTTTGGGTAAAAACACAGCTCCTGCTGTGACCATCGAAACTGAAAGGGCCCGTCAAGCCAACTACATCCAAAGCGAC
Seq A exon
AACTTCTACAAAGAGGCCAATAAGTCATTTATGCCCACTGGATACTCTCTGCCTTACGACACTCCACTCAACAAACAAGCCAAGGCCAACAGCATCGTCACCAGCAAT
Seq C2 exon
GTCAAATACAGGGAGAAGTACATCAGCACACTGGGCACCTGGAAGTCCATTCCTGACCGCCCTGAGTTCTTCCACAGCAGGATTGTGAGGGACTGCATCAGTGAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630_MULTIEX3-9/26=8-19
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.028 A=0.056 C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(33.3=25.0),PF0088013=Nebulin=PU(59.3=44.4)
A:
PF0088013=Nebulin=PD(33.3=25.0),PF0088013=Nebulin=PU(55.2=44.4)
C2:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAACTACCGTCATGACTATGCCA
R:
TCACTGATGCAGTCCCTCACA
Band lengths:
210-318
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]