DreEX0050614 @ danRer10
Exon Skipping
Gene
ENSDARG00000075707 | nid2a
Description
nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GENE-030827-2]
Coordinates
chr17:14815803-14828394:-
Coord C1 exon
chr17:14828248-14828394
Coord A exon
chr17:14818311-14818439
Coord C2 exon
chr17:14815803-14815928
Length
129 bp
Sequences
Splice sites
3' ss Seq
CAGTTTGTGTCTCTCTGTAGATG
3' ss Score
10.16
5' ss Seq
AGGGTAAGA
5' ss Score
9.21
Exon sequences
Seq C1 exon
CTGTAGATCCCGAGCCAGAAAACGTAAACCCCTGTTACTCTGGAAATCACGACTGTGATACAACGGCTCGATGTGTGCCAGGTGAAGGACAGCTGTTTTCTTGCCAATGTGCCACAGGTTACAGAGGAGACGGACGCAACTGTTATG
Seq A exon
ATGTTGATGAGTGTGCAGAAGGTCTGAGCTCTTGTGGTGCTCACTCTCACTGTGTGAATCTGCCTGGAAGCCATCGCTGTCAGTGTGAGAGCGGATTCCAGTTTGGATTTGATGGACGCACATGTCAGG
Seq C2 exon
ATGTGGACGAGTGTGGTGAGCAGCCGTGTCACGCCCAGGCCTTGTGCTCAAACAGTCCGGGATCTTTCCGGTGCCAGTGCCAACCGGGATACCATGGAGATGGTTTCCAGTGCCACCCTCAAAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000075707_MULTIEX2-2/3=1-3
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.020 A=0.000 C2=0.023
Domain overlap (PFAM):
C1:
PF129472=EGF_3=WD(100=74.0),PF0764510=EGF_CA=PU(0.1=0.0)
A:
PF0764510=EGF_CA=WD(100=95.5)
C2:
PF129472=EGF_3=WD(100=79.1)
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGATCCCGAGCCAGAAAACGT
R:
CCATCTCCATGGTATCCCGGT
Band lengths:
246-375
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]