DreEX0065103 @ danRer10
Exon Skipping
Gene
ENSDARG00000052997 | sema4e
Description
semaphorin 4e [Source:ZFIN;Acc:ZDB-GENE-990715-7]
Coordinates
chr2:49864910-49869386:+
Coord C1 exon
chr2:49864910-49865079
Coord A exon
chr2:49866995-49867047
Coord C2 exon
chr2:49868653-49869386
Length
53 bp
Sequences
Splice sites
3' ss Seq
CTGACTTGTTTTTTCCACAGCAC
3' ss Score
9.6
5' ss Seq
TAGGTAAAT
5' ss Score
6.62
Exon sequences
Seq C1 exon
AAGCAGCTGTATGTGGGTTCGGAGGTGGGCGTGGTTCAGCTGTCAATCAGTGAATGTGGGCGGTACCAGACATGTCTGGACTGTGTTCTGGCCAGGGATCCGCACTGTGGCTGGGACCTCGATACTGAGCACTGTGCTACTATTAACAGCATCCACAGAACCAGAAGCAG
Seq A exon
CACTGTGATTCAAAGCTTAAATGATGGAGATGCCAGTCAATGTCCAGCAATAG
Seq C2 exon
GTGTCAGTAAACCAGTCAACATCTCCTTTTACCACGGGAATACTGTGAAGCTGGGCTGCCAGCCTTATTCCAACCTGGCGCAGGTTGAATGGCAGTTAAACGGGGAGCCAATCAAACCATCAAATACCATCCAGATCCTCTCCGATGGCTTGATGATATTCAACGCATCTTTGGACGCCACCGGCTACTACATCTGCAGCTCCATCGAGACCGTCTCCCAGCGCAAATACCAGACTCAGCATGTAGCGTATGATGTAAAGATGTGGTCCGAATCTGGAACCACAGCGTCACTTCATCATGTTAAGGAAAAAGAGCGCACCCTAGTGGCCATGGTGGTGATACTGTCACTAGTTCTAGCTGCATTGTTGATATGGAACCTGTACAAAGGCCATTTGTCTCTGCCATGTTTACACAGGAGGGTAAAAGACACGCAAAACAGGCACGAAGACGATGCTAACCAGGTTCAGCCACAGAGACTAGCATCATCGGTAGTGAACTTC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000052997_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.234
Domain overlap (PFAM):
C1:
PF0140314=Sema=PD(5.3=42.1),PF0143720=PSI=PU(70.9=68.4)
A:
PF0143720=PSI=PD(27.3=78.9),PF138951=Ig_2=PU(0.1=0.0)
C2:
PF138951=Ig_2=WD(100=41.0),PF054346=Tmemb_9=WD(100=57.1)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGGGACCTCGATACTGAGCA
R:
CCAGGTTGGAATAAGGCTGGC
Band lengths:
138-191
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]