DreEX6008620 @ danRer10
Exon Skipping
Gene
ENSDARG00000099045 | cacnb2a
Description
calcium channel, voltage-dependent, beta 2a [Source:ZFIN;Acc:ZDB-GENE-080808-7]
Coordinates
chr7:71622995-71626613:-
Coord C1 exon
chr7:71626477-71626613
Coord A exon
chr7:71625259-71625335
Coord C2 exon
chr7:71622995-71623128
Length
77 bp
Sequences
Splice sites
3' ss Seq
TTTGTTCATTTTGTTCACAGTAA
3' ss Score
7.09
5' ss Seq
CAGGTAAGA
5' ss Score
10.77
Exon sequences
Seq C1 exon
AAATTTAATAACGACTGGTGGATCGGCCGCTTGGTGAAGGAAGGCTGCGACATCGGTTTCATCCCAAGTCCAGTGAAGCTGGAAAGCAGTCGCCTTCGCCAGGAGCAGCGCGCAAAACAAGGACGCTTTTACTCAAG
Seq A exon
TAAATCAGGGGCAAACTCTTCATCCAGTTTAGGGGACATTGTTTCAAACTCACGGAAATCAACTCCTCCCTCCTCAG
Seq C2 exon
CTGTGGACATTGACGCCGCAGGTTTAGATGCAGAGGAGAGCGATCCTCCTCCCCATCAGCGATCTCCCAAAGCCAGCCCAAACACAGTCATATCCCCTTTAGCCCGAGAGAAACGAATGCCTTTCTTTAAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099045-'5-11,'5-9,6-11=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.338 A=0.764 C2=0.813
Domain overlap (PFAM):
C1:
PF0001823=SH3_1=PD(43.1=47.8)
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTGAAGCTGGAAAGCAGTCG
R:
CGTTTCTCTCGGGCTAAAGGG
Band lengths:
181-258
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]