DreEX6008939 @ danRer10
Exon Skipping
Gene
ENSDARG00000063126 | gusb
Description
glucuronidase, beta [Source:ZFIN;Acc:ZDB-GENE-070705-256]
Coordinates
chr5:1820238-1827937:-
Coord C1 exon
chr5:1827794-1827937
Coord A exon
chr5:1820728-1820906
Coord C2 exon
chr5:1820238-1820384
Length
179 bp
Sequences
Splice sites
3' ss Seq
ATGCTGCTGTTTGCGTGCAGATT
3' ss Score
8.56
5' ss Seq
CATGTAGGA
5' ss Score
1.81
Exon sequences
Seq C1 exon
GTTCAGGCGATCGCAGAAGACGGACAGACGGATATTTACACTCTTCCTGTGGGCGTTCGTACTGTTCAAGTCACCAGATCCAAGTTTCTCATCAACAACAAGCTGTTTTACTTCCACGGGGTCAACAAACACGAGGATGCAGAC
Seq A exon
ATTCGAGGGAAAGGCTTCGACTGGCCTCTGGTGGTGAAGGATTTTAACCTGATGAAGTGGATGGGAGCCAACTCCTTCCGCACCAGCCACTATCCGTACGCTGAGGAGATCCTGCAGACGGCGGATCGACACGGCATCGTCATCATCGACGAGTGTCCGGGAGTCGGCATTAAAGACAT
Seq C2 exon
ACGCAGTTTCGGTAATGTTTCTCTGGCTCATCATCTGACCGTGATGGAGGAGCTGGTCAGACGTGATAAAAACCATCCGTCTGTGGTCATGTGGTCGGTGGCCAATGAGCCGGCGTCTGAAATGCCGCCTGCGGGACTCTACTTTAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000063126-'6-7,'6-6,7-7=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0070316=Glyco_hydro_2=PD(19.2=39.6),PF0283612=Glyco_hydro_2_C=PU(8.6=54.2)
A:
PF0283612=Glyco_hydro_2_C=FE(19.5=100)
C2:
PF0283612=Glyco_hydro_2_C=FE(16.2=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATCGCAGAAGACGGACAGAC
R:
TTTCAGACGCCGGCTCATTG
Band lengths:
258-437
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]