DreEX6013877 @ danRer10
Exon Skipping
Gene
ENSDARG00000029372 | rasa4
Description
RAS p21 protein activator 4 [Source:ZFIN;Acc:ZDB-GENE-060526-349]
Coordinates
chr5:58671935-58691766:+
Coord C1 exon
chr5:58671935-58672026
Coord A exon
chr5:58687781-58687898
Coord C2 exon
chr5:58691613-58691766
Length
118 bp
Sequences
Splice sites
3' ss Seq
ATTCTGTTCTTCTCTCGTAGAGG
3' ss Score
9.02
5' ss Seq
AACGTGAGT
5' ss Score
9.54
Exon sequences
Seq C1 exon
GTCCTGTTCAACATCAATAAGCTTCAGTCTGCACTGCAAGAGGAGGGCTGGTTCCGCCTGGGGCCTGACAAATCTAAACACAGCGAATATGA
Seq A exon
AGGTACTCTGGGCTCCCTGAGGCTGCATCTCCGGCTTCGAGACGAGGTTGTGTTGCCATCCAGCCATTACAAGCCACTCACAGAGCTACTCAGCCAGTCGGTCGGCTCTCAGCTCAAC
Seq C2 exon
GGAAACTGGCCTGATTTCATCATGCTGATTGATGAGACCACTACAGCGGAGAGCAGACAGGAAGTAGCTAATAATCTAGTAAAGCTGTTTTTGGGTCAAGGGCTTGTCAAGGAGTTCTTGGACGTTCTGTTCAAACTGGAGCTGGACAAGACTA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000029372-'8-11,'8-9,10-11=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0016825=C2=PD(1.2=3.2)
A:
NO
C2:
PF0061614=RasGAP=PU(6.8=25.0)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCCTGTTCAACATCAATAAGCT
R:
AGTCTTGTCCAGCTCCAGTTTG
Band lengths:
245-363
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]