DreEX6020289 @ danRer10
Exon Skipping
Gene
ENSDARG00000071213 | rgl3a
Description
ral guanine nucleotide dissociation stimulator-like 3a [Source:ZFIN;Acc:ZDB-GENE-101130-1]
Coordinates
chr6:7985905-7989525:-
Coord C1 exon
chr6:7989277-7989525
Coord A exon
chr6:7986106-7986209
Coord C2 exon
chr6:7985905-7985989
Length
104 bp
Sequences
Splice sites
3' ss Seq
TTTTATTTGTAACATTACAGGAG
3' ss Score
6.87
5' ss Seq
CAGGTAATG
5' ss Score
9.43
Exon sequences
Seq C1 exon
GAACTTTTTAGCAAAGTGGTCCCATTTCACTGCCTTGGCTGCATTTGGTCTCAAAGGGACAAGAAGGAAAACCAGAATCTAGTGTCCACAGTCCGTGCCACCATTGCTCAGTTTAATGTGGTCACTAACTGCGTTATCACCTCGCTGTTGTGTCCTCCTGCCACCTCCCCTCCTGGATCCCCGTCATGCCATCGCAGCAGCCCTACATACAGAGCAAAGATTATCGAGAAGTGGATCTCTGTGGCTCAG
Seq A exon
GAGTGCCATCAGCTCAGAAACTTCTCCTCTCTAAGAGCCATCCTGTCTGCTCTTCAGTCAAATGCTGTCTACCGGCTGAAGAAAACCTGGGCTGTGATCAGCAG
Seq C2 exon
GGAGAGTATGGCTGTCTTTGAACACCTCTGCGACACCTTTCCTGATGAGAACTGTGTGCTGATCAACCGAGAGATCTTAGTAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000071213-'16-18,'16-17,19-18=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.048 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=FE(35.8=100)
A:
PF0061714=RasGEF=FE(14.8=100)
C2:
PF0061714=RasGEF=FE(12.2=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTTGGCTGCATTTGGTCTCA
R:
GGTGTCGCAGAGGTGTTCAAA
Band lengths:
254-358
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]