DreEX6029204 @ danRer10
Exon Skipping
Gene
ENSDARG00000052997 | sema4e
Description
semaphorin 4e [Source:ZFIN;Acc:ZDB-GENE-990715-7]
Coordinates
chr2:49858698-49865079:+
Coord C1 exon
chr2:49858698-49858914
Coord A exon
chr2:49862337-49862452
Coord C2 exon
chr2:49864910-49865079
Length
116 bp
Sequences
Splice sites
3' ss Seq
TGCATTTTGTTTCGTGTCAGAGA
3' ss Score
7.06
5' ss Seq
AAGGTAATA
5' ss Score
8.49
Exon sequences
Seq C1 exon
TGCATTGACAATCATGCCAGAGAAAAGGGCATCACCAAATCTCTGGAGCTTCCAGACAAAACGTTACAGTTTGTCAAAGACAAGCCTCTGATGGACCAGGCCGTCACTGCAGAGCAACCCCTGCTGGTGAAGAGAGGAGCTGCGTTCACTAGAATAGTAGTGACCACCGCGACCGCCTTGAATGGGACCAGCCATCAAGTCATGTTCATCGGCACAA
Seq A exon
AGAGTGGATCAGTCCTGAAAGCTGTGAATTATAATGGAGAGATGGTCATTATGGAAGAGATTCAGCTATTTGAGCCCTCGGAGCCCATCAAGATACTGCGTCTTTCAAGCAGCAAG
Seq C2 exon
AAGCAGCTGTATGTGGGTTCGGAGGTGGGCGTGGTTCAGCTGTCAATCAGTGAATGTGGGCGGTACCAGACATGTCTGGACTGTGTTCTGGCCAGGGATCCGCACTGTGGCTGGGACCTCGATACTGAGCACTGTGCTACTATTAACAGCATCCACAGAACCAGAAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000052997-'15-21,'15-19,16-21=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.014 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(15.8=100)
A:
PF0140314=Sema=FE(8.3=100)
C2:
PF0140314=Sema=PD(5.3=42.1),PF0143720=PSI=PU(70.9=68.4)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAGAGAGGAGCTGCGTTCAC
R:
TGCTTCTGGTTCTGTGGATGC
Band lengths:
258-374
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]