DreEX6066995 @ danRer10
Exon Skipping
Gene
ENSDARG00000100991 | chrna3
Description
cholinergic receptor, nicotinic, alpha 3 [Source:ZFIN;Acc:ZDB-GENE-070822-1]
Coordinates
chr18:50641553-50643863:+
Coord C1 exon
chr18:50641553-50641609
Coord A exon
chr18:50642545-50642684
Coord C2 exon
chr18:50643819-50643863
Length
140 bp
Sequences
Splice sites
3' ss Seq
GTTGTGTGTTTCTGTTTCAGGGG
3' ss Score
11.5
5' ss Seq
GTGGTGAGT
5' ss Score
8.95
Exon sequences
Seq C1 exon
CCGGAGCTCTCCTGATCTTCTTCTCCTCATTTTCTCCTCTGTGTTTTCTCCTGTCGG
Seq A exon
GGGTTTGCTGCTCTGAGGCCGAGCACCGGCTCTTCTCTGTCATTTTCTCCAGCTATAACCAGTACATCCGACCAGTGGAGAATGTGTCTGATCCAGTGGTCGTCCAGTTCGAGGTCTCCATGTCACAGCTGGTCAAAGTG
Seq C2 exon
GACGAGGTGAACCAGATTATGGAGACCAATCTTTGGCTGAGACAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100991-'0-3,'0-2,2-3=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0293118=Neur_chan_LBD=PU(18.8=83.0)
C2:
PF0293118=Neur_chan_LBD=FE(6.8=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAGCTCTCCTGATCTTCTTCT
R:
CAAAGATTGGTCTCCATAATCTGGT
Band lengths:
90-230
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]