DreINT0014419 @ danRer10
Intron Retention
Gene
ENSDARG00000074808 | MEGF6 (1 of 2)
Description
multiple EGF-like-domains 6 [Source:HGNC Symbol;Acc:HGNC:3232]
Coordinates
chr11:41108235-41110383:-
Coord C1 exon
chr11:41110282-41110383
Coord A exon
chr11:41108364-41110281
Coord C2 exon
chr11:41108235-41108363
Length
1918 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
3' ss Seq
TTACATCTGTGTGTGTATAGTCT
3' ss Score
6.29
Exon sequences
Seq C1 exon
CATGTCCAGCGGGTCGCTATGGTCTGGACTGTGCTCATGTGTCAGCGTGTGGGACAGGAGTGAGGAATGATCCGGCTACAGGAAGATGCACTGCACATCAAG
Seq A exon
GTGAGCTGATGGTGCTTCTCTCTGCAACACCAAAACCCAACTCAGTGTTTGTGCTGCAAAACTCCTCTTACTATAAAACACATTTTACTGGACTGTCCTGCTTTTACTGATTCCAGAAGACTTTTTTTATGAAATGAACTCTTTTAAGGACATTTTTAACAAAATGAAACCAGAAAAGATATTAGATTTTTTTTTATCATGTATTAACACAAAAAATCTTGTTTAATTTATGTTTTATTTTGTTTGTTGATTTTTAGGGCACTATCATACACCCGGCGCAGTGTGGCGCAAGGCGTGACGCTGTAGTCTTTTGGTAGTTTCAGCTTGGCGCAAGAGTCGTTTTGAGGCGTTGCGCTACGCTGTTTAAATAGCAAATGCATTAGCGCTCATTTGTGCGCCCATAGGCGTTCTGGTCTAAAAAGGAAGCGCTCTGAGGCGGACCGCTGGCGCGTTGCTATTTTGAGAAACTATAATAGATTTTTCATTAGACCAAAACTAACCCGGTCTAAACTCCAGCGCAGAGTTGCGCCTCGCTTACACACTGCTTAATACACACAAGAGAGCAATAGGCAAATATCTTTACATATGAAAAAATGTAAATATTAATGATATATATAGGATATAATAAGAATAGATATAGAATATAAATATAAAGAATTAAAATATTACAAAACATTTTATTTTCTAGCCTACATAAATATGAAAAATCACTGCTTTTATGTCTTCTTCATCTCTGGGGGCTTTTTCAGTTTATTCATAACAATTTACTTTTGTATAATGTTATTATTATTAGCAGTATTATTTATTATATCCATATTTATATTTGTTTTATTAAAAACAAGCTTAGATTTGGCCACCTGTCAGGTTTTAGACTATATGGGGCATGGCAGGTGTATATGGAAATAACTCAGTATTTTGACCACACTTGGTTATTATTGTTCATTTATTCGTTTGCTGGAAATTAGAACTGAATTTAGAAATAGTTTTGAAACAAAATTTTGCGCTTAACAAACGAAATTATTTATTTATAGACTAATTGATGTCTGTGCGTAAAGGTTTCCCTATCCAAGAGTGAAAGTGAAAGTAGATCCATTATCTCTCATTCTCACGCAGTAGATGCTCTGTTTAACAGTTTTCTGTTAATAAAATGTTAACTGTTTGCTAGTGAAATGCTCATTTTTTCCATTAGACTTACTTTGCGTCCTGTAAATAGCGAATGTGCTCTTGGCGCGACGTAGCTGGCTCTTAAAGGGAATGGGAGATGAGACTCTAATTGGTTTATTCTCAAAACACACCTATAACTCACTAAGAAAATAAACTCAACCCTTTTATAACATGCGCCTTGGCGCAAAGCAGGTTTTCCCGACTGTAAATTAGCAAAAATGCGTTCTGACACGCCCTGAAAGCGTTTGCGCCCTGCGTTTTGCGCTCTGCGCATGGACCGTCAAAATAGAGCCCTTAAATTTTATGTTTATTGTTGAAAGATTCCTGCCATGAAAATAGCCTTGGTTGCTGACATGGCATTAAATAAAAAGCACATCACATTACTCTCTGCAACACCAGCAGGGAAACAAAAGTCTCAAAGCATGCCAAATGTGATCGTCAGCATGCACTGTTACACTGGGATGTTTTCTTGACCTAGTAAGAACTCTGGCTGAAGTAACTGAAGTTTATTAGTAGGGGCTGCTGGGCGAACAGAGCACAGAGCATTGCAGTAATCTAATCTAGAGGTCATAAATGCATAAGCTTTTCTGCATTTGAGATTTCCTAGCTTAGCGATATTTCTCAAATGAAAGAAGGCTGTTTTTATGATATGGGAACTATAATTTTAAAGCTTAACTAGCTGTCTAATATAACACTGAGGACAGTAGAACTAATACTTATATTGTTTCCCTCTGTTACATCTGTGTGTGTATAG
Seq C2 exon
TCTGTCCTCCAGGTTGGTTTGGTTCTGGCTGTTCTCAGCGTTGTGACTGCAGTAACAGAGGTGTGTGTGACGGAGTCTCTGGAAACTGCTCATGTGCTCTCGGCTGGACCGGCAAACACTGTGATAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000074808:ENSDART00000109204:32
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF126612=hEGF=WD(100=29.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGGGTCGCTATGGTCTGGA
R:
CTTTATCACAGTGTTTGCCGGT
Band lengths:
222-2140
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]