DreINT0025512 @ danRer10
Intron Retention
Gene
ENSDARG00000014674 | acsl3b
Description
acyl-CoA synthetase long-chain family member 3b [Source:ZFIN;Acc:ZDB-GENE-030131-492]
Coordinates
chr2:47642403-47643873:+
Coord C1 exon
chr2:47642403-47642580
Coord A exon
chr2:47642581-47643763
Coord C2 exon
chr2:47643764-47643873
Length
1183 bp
Sequences
Splice sites
5' ss Seq
CCTGTGAGC
5' ss Score
3.28
3' ss Seq
ATGATCTGAATGGTTTTCAGTGG
3' ss Score
5.46
Exon sequences
Seq C1 exon
GTGATTTTGGGCGATTATAACTGGTTGTCGTATCAAGACACTTTCCATCTGGCTCAGAGGTTTGGAAGTGGACTGGCTGCGCTGGGTCAGAAGCCTCTCTGCAACATCGCTATTTTTTGCGAGACTCGAGCTGAATGGGTCATAGCGGCACAGGCTTGCTTCATGTACAATTTCCCCT
Seq A exon
GTGAGCATTCGGAACACACACACACACACACACACACACAAACACACACACACATACATAGTGTTGCCTCCAATAGCTGCCATATTGTGCTGACTAGGAAAAAATATTTGGCCAAAAATAGCACTGCTAGGGTGGTTGATGCTAGACAGTATCACTGAAATCAGAGCATTTACTCTCTTGAAATTGATACTATACATACAAAATATGCATATATATATATATATATATATATATATATATATATATATATATATATATATATATAAAAAAATATATATATATAAAAAAAAAATATATATATATATATGTTATATGTTAATTTCTTATAACTATAACTATTTATATTATTTGTTATATTCATTTACTATCCCTCGATATAACGCGGCCCTGCCTCGTAGTTCGCCAAATTTTTTTTTGTGCAGTTTGACATGCTTTTTTTAACAGCGCATTGTGTGCTACATCCTGATTAGCTGTAAACCATTGTCAATCAATCTCCTCATTGTCTCCTCTATAGTACAGAATGCATTCAGCTTGCAGAATTAACATAAATCTTCGATCGCTAGCAGTATGAGTTCCAACAAGGATGCAAAAAGGGTTGTAACTGTCTGACTACTTCAGATTTTGCCTATCACAGGTTGTTTTTAGAACTTAACTCCCGCAATAAATTAGGGACCACTGTATTACTAACAGTTATATGGTATAGTCCGATTGAATGTTCTTCTAACATGCACTCAAAAAATGACGTTTGCTTTTTGTTCAACTACTAATTTAAATTGAGCTAAAACAACACAATTCTTGAGCTTTTTGGGGGAGGGACAACGTAATTTGGTTAAAAATTAATAAGTTAACTTAATTCCTTCATGTTGTTGTCCCAGTGTACTAAATAATCTCTTACAACATATTCAAATATATACTGGATATATACTTACATTTTGTGTACATGAATGCTTTTATTTCAGTATATGTGATAAGTTTTTGTGGTCTATTTTAATGCTTTTGTTGTTGTTTGTGAGACACTTTGAGTTGCATGTATGTTGGAAAAGTGCTATACAAATAAAGTTTTTTATTATTATTATTATTATTAAATATTTTTATCTTCATCATTTTGGTGGAAATAATGGATATAAAATATACCTTTCACTTATTTCTATCTAAAGACACTAATGATCTGAATGGTTTTCAG
Seq C2 exon
TGGTGACTCTTTACTCTACACTGGGAGGATCAGCTATAGCTCATGGTCTGAACGAGACTGAGGTCACACACATCGTCACAAGCAAAGACCTGCTGCAGAGCCGCCTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000014674:ENSDART00000141974:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(12.4=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(7.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGATTTTGGGCGATTATAACTGGT
R:
CTTGAGGCGGCTCTGCAG
Band lengths:
288-1471
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]