DreINT0025723 @ danRer10
Intron Retention
Gene
ENSDARG00000001431 | actn3b
Description
actinin alpha 3b [Source:ZFIN;Acc:ZDB-GENE-030131-2040]
Coordinates
chr7:6886725-6888459:+
Coord C1 exon
chr7:6886725-6886904
Coord A exon
chr7:6886905-6888312
Coord C2 exon
chr7:6888313-6888459
Length
1408 bp
Sequences
Splice sites
5' ss Seq
GAGGTACAC
5' ss Score
5.99
3' ss Seq
CTTCTCCATGTCTCCTCCAGCAC
3' ss Score
8.93
Exon sequences
Seq C1 exon
GAGATCGGTCATGTGTCGGTGGACATCGCCGGCTCTCTGGAGGAACAGATGAACAACCTGAAGCAGTACGAGCAGAACATCATCAACTATAAATCCAACATCGACAAGCTGGAGGGAGACCACCAGCTCATTCAGGAGGCGCTCATCTTCGACAACAAACACACCAACTACACCATGGAG
Seq A exon
GTACACACACGCACACACACACACACACACACACACACACACACACACATAAAGAGTGACACACACAGACCTACGCACACAAACAAAAATACAGATGTTTGTTATTGTGAATTGTGGGGACATTCTATAGGTTTTAATTGTTTTATACTGTACTGTATTTTCTATAACCCTACCCCTTAAAGGGAAATGTGCAGTTTTATTTTCTGAAAACAGAGCTTCTGGAGAAATTGGGACATCAGCAATTTCTTAATTCAACATCACCTTATAATACCTGTGTCACACTCTATGTCATTATACACATTTGTGTCCTGATACATGTGTCATAAACACACACACACACACACACACACACACACATACACACATACATTTTTTTGGTTTGTTTTGTGTTTTTGTTTTGTTTTTAGTTTTGCTTTTGTTTTTAGTTTTTTTTGTGGGTTTTTTTGGTTTGGTTTTTGTTTAGTGTTTTGTTTTGTTTTTAGTTTTGCTTTTTTATGTTTTTTGTGTTGTTTTTGTTTTGTTTAGTGTTTTTGTTTTTTTTTGTTTAGGTTTTAGTTTTTTGTGTGTTTTTTGTTTTGTTTTTGTTTAGTGTTTTGTTTAGTTTTTAGTTTTGCTTTTGTATGTTTTTGTGTTGTTTTTTGTTTTGTTTAGTGTTTTGTGTTGTTTTTGTTTTGTTTTTAGTTTTTTGTGTTTTTTTTTCCGTTTTGTTTTTGTTTAGTGTTTTATTTTTAGTTTTGCTGTTTTATGTTTTTTGTTGGTTTTTGTTTTGTCTTGTGTTTTTTGGGTTGTTTTTTGTTTTGTTAGTGTTTTTTGTGTTGTTTTTTGTTTTGTTTTTAGTTTTTTGTGTTGTTTTTAGTTTTGCTTTTTTATATTTTTTGTGTTGTTTTTTTTTGGTTTTCTTTTTGTTTAGTGTTTTGTTTTAGTTTTGCTTTTGTTTAGTGTTTTTAGTTTTGCTTTTGTTTAGTGTTTTTTGAGTTGTTCTTTATTTTATTTAGTTTTTTGTTTAGTTTTTTTTTTTTGTTGTGTTTCGTACTTCAAGGACAACACACACTTAATGCACAAATACACTTCATTGAACATCTACACATAGACATCACCTATGCTATATAACTGCAGGTTATGTTTTTAACTGCAGGTTTTAGTTAAAGTCATTAGTAATTAATAAAGTTATGCTAACTAATAACCATAACTTTATTACCACCATTTGTAAAGCTGCTTTGAACCAGTAATTATTGTGAAAATCGATATACAAATTTCCCAATACTGGGTTGCGGCTGGAAAGGGAATCCGCTGCGTAAAACATATGCTGTAATAATTGGCAGTTCATTCTGCTGTGACGACCCCTGATAAATAAGGAGCTAAGTGAAGGAAAATGAATGAATGAATGACTTCTCCATGTCTCCTCCAG
Seq C2 exon
CACATCCGTGTGGGCTGGGAGCAGCTGCTCACCACCATCGCTCGCACCATCAACGAGGTGGAGAACCAGATCCTGACCCGCGACGCAAAGGGCATCAGCCAGGAGCAGCTCAACGAGTTCAGAGCCTCCTTCAACCACTTCGACAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001431:ENSDART00000001649:17
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (disopred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=FE(56.7=100),PF088696=XisI=PU(65.2=71.7)
A:
NA
C2:
PF0043516=Spectrin=PD(20.2=42.9),PF088696=XisI=PD(31.8=42.9),PF134991=EF-hand_7=PU(33.3=53.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGATCGGTCATGTGTCGGTG
R:
CCTGTCGAAGTGGTTGAAGGA
Band lengths:
327-1735
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]