DreINT0041114 @ danRer10
Intron Retention
Gene
ENSDARG00000052748 | capn1b
Description
calpain 1, (mu/I) large subunit b [Source:ZFIN;Acc:ZDB-GENE-060503-491]
Coordinates
chr22:26284124-26284337:-
Coord C1 exon
chr22:26284280-26284337
Coord A exon
chr22:26284189-26284279
Coord C2 exon
chr22:26284124-26284188
Length
91 bp
Sequences
Splice sites
5' ss Seq
AACGTAAGC
5' ss Score
7.35
3' ss Seq
TGCTCTCCTGTGTTCTGCAGACC
3' ss Score
10.55
Exon sequences
Seq C1 exon
GACATGGAGATTTCAGTTCACGAGCTCCGGACCATTCTGAACCGAGTGATGACAAAAC
Seq A exon
GTAAGCTCAGAGGACGTTTTTAAAAAATTCAGATCTGATGTGTTTACATTTTTGTGCTCGATAATTACCTCTGCTCTCCTGTGTTCTGCAG
Seq C2 exon
ACCGAGACTTAAAGACAGATGGGTTCAGTTTGGAGTCTTGTAGGTGCATGATCAACCTGATGGAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000052748:ENSDART00000105099:14
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF138331=EF-hand_8=FE(31.1=100)
A:
NA
C2:
PF138331=EF-hand_8=FE(34.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGGAGATTTCAGTTCACGAGCT
R:
TCCATCAGGTTGATCATGCACC
Band lengths:
119-210
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]