DreINT0042121 @ danRer10
Intron Retention
Gene
ENSDARG00000023645 | ccbl1
Description
cysteine conjugate-beta lyase, cytoplasmic [Source:ZFIN;Acc:ZDB-GENE-040426-2676]
Coordinates
chr5:32666257-32668124:-
Coord C1 exon
chr5:32668038-32668124
Coord A exon
chr5:32666964-32668037
Coord C2 exon
chr5:32666257-32666963
Length
1074 bp
Sequences
Splice sites
5' ss Seq
AAGGTATTA
5' ss Score
7.04
3' ss Seq
TTTTCTGTCATCATTAATAGGAG
3' ss Score
8.49
Exon sequences
Seq C1 exon
GGACTGGCCACCATCCCGGTATCTGCTTTCTACAGTCCAGAACACAGAGACCAATTCCAAAAATACATCAGATTCTGCTTTGTTAAG
Seq A exon
GTATTATCCTCACATAATATGATGCAGAGCATGAAATGATTAAAATAGATATTTTAAAAATGTTTTACATAATTGCTTAATTTTCTGAAGTGTCAGTATTATTATAATATTAACCCCAAATATACAGTATAGATATCTAATGGGCATACTTGGTTCTAGTTTTATGTTTAAATTATTTTTGTATTTTATGTTTTTAGACAATTAGTAACAATAATTATTACAATTCTAAGTCCATTAATTTTAGTTAAACCTTTAGTATTGTTTAGTGCTTTTGTAAACAGTTTTTTTAAATGTTGGTATTATATTTAAGATACATATAATATAATATAATATAATATAATATAATATAATAAAATATAATATAATATAGCCATATCACACAGCCACAGCCATATAACCCTGCAGCCCAAGATCGGTTATTCACTGAAGCTAAGCAGGCTGAGCCTGGTCAGTACCTGGATGGGAGATCACATGGGAAAGCTAGGTTGCAGCTGGAAGTGGTGTCAGAGAGGCCACCAGGGGGCACTCAACTTGTGGTCTGTGTGAGTCCTAAAGCCCCAGTAAATAGTGAAGAGGACACTACACTGTCAGTGGGTGCCATCTTTCGAATGAGACGTTAAATCGAGGTCCTGACTCTCTGTGGTCATTAAAAATCTCATGGCACTATTAAAGAGTAGGGGTGTTACCCAGGTGTCCTTGCCAAAGTCCCTCAATCGGTCCTTAAGATCATGGCCTCTCAATCCTCCTCGTCCTCTAAATTGACTCTCACGGTCTCTCCACTCCCCCAGTAGCTGGTGTGTGGTGAGCGTACTGGCGCCATTGTCCTTTGGCTGCCGTCGCATCATTCAAGTGGATGCTGCACACTGGTGGTGGTGTGGAGAGACCCCCCTCATGATTGTAAAGCACTTTGGGTGTATGGCCATACACAATAAATGCGCTATATAAATGCACATTGTATTACATTACATAATATACAAAATTTAAAGTTGCTATTATAATAACAATCATCATATTTTTATTATGTTAAAATGTAAAAATACTTAATATCTTTACATTTTCTGTCATCATTAATAG
Seq C2 exon
GAGGACTCGACCCTGCAAGCAGCAGAGAACATCCTAAAACAGTGGAGTGAAAACAAATGAAAAAATCCTGTCAGATTAAAACTGCACAGACAGTCTGTTTGGCTTATGAATAGTTAGCGAAACGTGTTTGATGATGTTATTGGGCTTAAGGTTGAATATGCTTGCAGGATTAGCAGATGGTGTAAAAACTTTTAGGGGATTTTTGAGCAAAAAAATTAAATCTATGTTCTAAATCCATGAATCATTGCTGCCATGAAAACAATCATTACACAATTTTCTTCCCTAAACAAATAGTATAGGTGGAATTTCTCTCAGTCTTTTAATCATTATTATCCTTTGGTTGTAATGTTCTCGATTTTCTTGTTTGTCTTCAAGTAGCCACATATTTTCTTCCATTTGTTCCGTTCTTGGATTATACTTTAATCTGATTATACTTCATAATGTCTGTTATGACCCTCTGGTTCCAGTCCATGTAAGAAATTACACATTAATATTTTTATATAACACCCTGTATTATGTTCAGCCATTTTTAGTAAAGGTCCTGTAAACTACCTCATACTTTTGCAAGAAAATCAAAACACAAATCTGTACTTTCTCAGTTCTTCAGAAGAAAGCAATTATGAGGCATTGAGTCACACACTGGTTTGTTTTGAAAACGGAACATGCTTTGCCGATTTCAGCATGGGAATAAAACAAATGTGAATGAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000023645:ENSDART00000004797:13
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0015516=Aminotran_1_2=FE(7.3=100)
A:
NA
C2:
PF0015516=Aminotran_1_2=PD(2.6=50.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCATCCCGGTATCTGCTTTCT
R:
TGATTGTTTTCATGGCAGCAATGA
Band lengths:
342-1416
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]