DreINT0045188 @ danRer10
Intron Retention
Gene
ENSDARG00000058000 | cep350
Description
centrosomal protein 350 [Source:ZFIN;Acc:ZDB-GENE-030131-6975]
Coordinates
chr8:14542891-14543480:-
Coord C1 exon
chr8:14543347-14543480
Coord A exon
chr8:14542996-14543346
Coord C2 exon
chr8:14542891-14542995
Length
351 bp
Sequences
Splice sites
5' ss Seq
CAGGTATCA
5' ss Score
7.33
3' ss Seq
TTTTGGATCTTCTCCAGTAGTTG
3' ss Score
2.9
Exon sequences
Seq C1 exon
ATGACTGAGCTGGCGCGTTCACAGATTGTAGGAGCACTAAGTGTTCCTGGACCCTCCATTACAGCCCTGCATGACCAGCAGAGACAGCACAGCCGCAGTTCAAAACCGCACCAGACACAAACCCACACAGACAG
Seq A exon
GTATCATCTCACCTCTGAGACAGATATTCTTCATAGTTGTTTACCCTCCTATTTCATGCTTGCTTTAGCTTATGAGCCAGATAGGCTTCAATAAAGGATTACCTCATAAGTAAATGAATATCTGCCATTATTATATGTCATTCCAATCATTGTGAAAGCTTACAAATGGGAAAGTACTGATGAGGAATTATTTAGAGATGAGTAGCTCATGACAAAATTTTTGTTTTTGGATGAACTATCCTTTAAACCGTGCCTTGCATTTACTAAAGTGAAATGTTTTTTCTAATTTGTCATAGTAGTGAATGCAATATATACATCAGTAATGTTACTGTTTTGGATCTTCTCCAGTAG
Seq C2 exon
TTGGAAAAGCGAAATGTCTCCTGAGAGACATCAGAGTCCATCAGAGGTCGAGACGCCACCAGACAGCCTCTCAGAGTCCATCCCTTCCAGAAGACCCACCATCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000058000:ENSDART00000146175:21
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGACTGAGCTGGCGCGTT
R:
TGATGGTGGGTCTTCTGGAAG
Band lengths:
238-589
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]