DreINT0045199 @ danRer10
Intron Retention
Gene
ENSDARG00000058000 | cep350
Description
centrosomal protein 350 [Source:ZFIN;Acc:ZDB-GENE-030131-6975]
Coordinates
chr8:14528855-14530830:-
Coord C1 exon
chr8:14530671-14530830
Coord A exon
chr8:14529016-14530670
Coord C2 exon
chr8:14528855-14529015
Length
1655 bp
Sequences
Splice sites
5' ss Seq
CTGGTAAGA
5' ss Score
9.45
3' ss Seq
TTTTTGTGTTCCTACCATAGAGA
3' ss Score
8.29
Exon sequences
Seq C1 exon
TCTCCTCCAGGGAAAGCCAGCCTCAGCTCTCTTCGACATTCAGACAGTAGCCGGCCTGGCACTGTCACTGGCACCAGCAGTTGCAGTAAGATGCAGGTGCACTCCGGCTCCAAGACCAGCGAGCCCCACACCAGCGATACTCATTCGGCCACTCCGTCTG
Seq A exon
GTAAGATCCTTCATTCTCCTATCAGATTTTAGAAGTTATCTGGATTGAGTTAAGATCTCTTTTCCTTTTAGGTTGCAACTTCTAGATTACACTACTTTGTTTTTTTTACATTTAGATCATAGTTCTATGGCTATGCCTGTTAGGCCTAATTATTTAGCAACAGTTTACCCTTTACATATTTACAAATAGGAGATGCAAAATGGAGGAGGAACCCTGTGGCAACAATATATAATGCTTGGTGTTCAGCTTTGGGTGTGCACTTCTCTTGATCAAACCACTTAGTCATGTTATTAAAGCACAGACAAGTCTACTTATTATGATGATCCATGAGAAATGGTGTCTTTGTTCCTTTTGTGGTTCAAATTGTTTAAATGTTTTAGTTGTGTACTTTTAATCCTTATGGAGACTGTAATATGTTTAATAATGTTTAATGCTACTGTGGTTTTACAAAGACAATAGTTGTATTTGTATGCACTCGTATGTTAAACCTATAATGCTTAATACGCTGGCGACATGCATGGTAATTTAAACATATTAAGGAGTTTTATTTGTGGTTTATTTTCTTTAAAAAAAAAAACAATTAGCACTTAGAGTTTTATAACCCATAACCCAGAATTCAGTTAGCATGTATGCACTTTAACCTGCACCTTAACCTCTTTTTAGTGCACATGTACAAGATGTAAAAGGAAATGTCCTTGCAGCAGATTTAAATTTGCATGTGCAGACCAAACAGGAAAATATATTTTGTAAAAGCCAAATGATGCAAGTTGATGGTAATCTCCACATATGAAAACATAGAATGAGAAGTCATAAAAACACTATGACATGAGATGATGATGATGATGATGATTATTATTATTATTATTATTATAAACTTTATTTTACCAGAAAAGACACGTTGAGATTAAAAATATCTTTTACTAGAGCGTCCTGGCCAAGATAGGCAGTATACTTGTCACATGGGTCTAACAGACAGCAAACAAGAAACAATTGACAGTTAACATGAATCACACATTGACAAAACAATTCAAAACATCTACAAGACTGTTTCAATTTCTAAATCATTTATACTCTTTTTAAAATCATTTAGTGAAATTAATTCTTTAAACTGCAACTTTGTTTGTAGATTATTCCATGCAAAAGTTGCTGCGTAAAAGCCTTTTTTCCCATCTCAATCCGCACTGTTAGGAACAGACAGTAAATTAATATCCTGTGACAGAAGGCCATAGCTAAGATCAGGTCTTTTCCAAATGTAATTCTGTAGATATGGGAGTAAATTTGTAAACAAGGATATGCCAATGCTTGTGCCTACGAATGGACAAAGCAGAACAACCTACTCTAATATACAGCACACAATGATGAATAAGGTATTTAAAATAATTTATAAATCTTAGTGCACCATGGTTAACAGTATCCAATGCATGCAGACTCTGAGATGAAGCATGCATATAGATAACATCTCATAGTCCAATTTCGAAATAAATGTTGCATTAACCAGCTTCTGTTTAGCATCAAAAAAGGCAGTTCTTAATTCTAAAATAGAATCCGATAACCTTAAAAAATAGGCATGTGAATGTGCACATTAATTGACAAAGTTTTAACATGCATTTCTTTGTAGAGATATTTTATAATATTTTTTTGTGTTCCTACCATAG
Seq C2 exon
AGACCACATCTGATCAGAGTGATATTGAGAGCCGCATTCACGCTCTCAAGGATGAGCTCCGCAAGCGCAAATCAGTGGTATACCAGCTAAAGAAGGAGCAAAAGAAAAGGCAAAAAGAGCGACTAAAGGCACAGGAAGCAAGTTTGCTGAAACAATTGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000058000:ENSDART00000146175:31
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=0.944
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTCCTCCAGGGAAAGCCAG
R:
CTCCAATTGTTTCAGCAAACTTG
Band lengths:
321-1976
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]