DreINT0045488 @ danRer10
Intron Retention
Gene
ENSDARG00000002365 | cers5
Description
ceramide synthase 5 [Source:ZFIN;Acc:ZDB-GENE-030131-1740]
Coordinates
chr22:5741493-5743647:-
Coord C1 exon
chr22:5743517-5743647
Coord A exon
chr22:5741551-5743516
Coord C2 exon
chr22:5741493-5741550
Length
1966 bp
Sequences
Splice sites
5' ss Seq
CATGTAGGT
5' ss Score
4.94
3' ss Seq
AGCTGTGTTTTTCTCTACAGGTG
3' ss Score
12.42
Exon sequences
Seq C1 exon
TCTCCAGACTCCAGGCATTCAGAAGGCCTGTCTAAACAGCTGGACTGGGATGTGCGAAAAGTCCAGCGATGGTTTCGACACAGAAGAAACCAAGACAAACCCAGCACAAGAACCAAATTCTGCGAGAGCAT
Seq A exon
GTAGGTTTCCAGTTGATCCTACTGTGCATGCCTGCTAGCGAATTAGCTTGTGGAGTAACATGAAAATATGCCAATGAATATCTGCATTCATAATCAAAACACAATGAGGAATTATTGGCATTACGACATGCTTCATGCTGATTTTTAGATTTTATGGTTGTTCATTTTGGTTTAATTATTTATTGTGTTCATTGTAAACTTTCACAGAGGTGAATTGTATAGGTTTATTTATTTTATTAAATGCACCAAGTAATTTATCAGCAATATTTGCTCGACAGCATGCTTCAAGTATTAAATATAAGCTTTCACTCTTTCTTTTTTAGGCACATTTCTCACTTTCGTTTAATATTTGGTGAATTTTTAATCTATTCATCATGTAGCATTTGGTGTTTCTTTGTAATCCGCTTGTTGAGTGGTGACGTGTTTGTGACGTATGTAATACCGTTTCCGGGTCCAAGCCGCCACTCATTTGAGTGGAGAAATCATTCGTTTATGATCACGTTTTATTCCTATCACACATTAAAGTTAATCTTATATAAAGTCATAGTGTACACAATTATCTCTGGGCTTGCTGCATAACAAATACCAAAAATGTAACAATATATAAAAAATTTATCACTTTCTGGCCATCGGATATTAGGCAATGGACATATATACTGCAATCGTGATTTTCGAAAGCATTAAATCGCTTTGTAAACGTTTATTATTACCATTTCATGAGCCGTCCCATTCAGTTGAATAGAGCGCTTGGACCTGGAAGCCGCTTCGCGTGACGTCACACTTAACAAGCGGATATGAAGGAAATTCCATTCAGAATGCATTGTGAAATGTAAAACTTTATTAATTTACTATACAGTATTTGTTATCAATTATTGTTTTATTAAGGGATTTATCAACATTATTTTCACTACAGCATGCGTTAAGTTTTAAGTAAATCTTGTAATTGCGTGTACTTTTTTGTTTTATGCACATTTTTAAATTTAGTTTCATTCACAAATTACCGTTTCTTCTTAATATAATAAAAATTCCACTCAAAACCCACCTTTTTCCATTTTTAGATGCATTTATCAAATAAAATAAGGAATCTATTAGTGACGTTTTATACGATACATTCACACGCCAGCTAAAAATGTTGTGGAGTTTGGTCGACTAAATACAAATTTTTCGATGGATAAATACTCTTGCTGACATCATCCCGTGTTAATGCGCATTTTGAAATATCGCCTGAGAAATGAGCGATGAAAAAGGCAAAAAAATATATAATAATCGATTAATTTGTAAAAAAACTACATAGTCCGATCACTTGAGATAGATTCGGACTTGTGCGATAAAGTTTGCTGAATTGCTGTTGCAAATTAAACTTTGACATTACACACACGTGACCAATCAGCAGTCTCCATTATCTTTTCCTTGTTTACTGTTGGTTGCTAGGTTACCAATACTACATTCGGCTGCTTTACTAACTTAACGGAAACACTCTTAATTCCCATTTGCATTGTTTATTTAGTTTTTTTACTTATTTATTTGATGTATGGTGAACTTTGAAAATATTTGCGTCACCTTAAGTTGGAAACCCAGCTACTGTTTTACCAAACAGCAATAAAAGAAATACAATTAGACTGATGATAAGACGTAATTTTGTTCATGCCGATATTAGCGGAATTGTTATAAAGTCACTAAATGCATATATTTGTATATTGCATACTGTTTAAAAGTATTACAATGTCAGAGTCATTAGTTGTCAAACAAAAAGTACACAACGAATCATTATTGAGAGAGCTGGTTGGTTTGGTTGATTAGCAGTATTAAATGAGGAATATTTACCGGATTTTAATGTAAAGGTTGAGTGGAGAGTAAATGAGGAAATACCAGTGTGATTATAATGCAATGTGATTCCTCTTATCTGTGTTTGAGTGTGTATGTTTAGTTGTTGTTTTTCTGACAACAGCTGTGTTTTTCTCTACAG
Seq C2 exon
GTGGAGGTTTACATTCTATCTGTCTATATTTACGTATGGGATGCGTTTTCTGTGGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000002365:ENSDART00000011076:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0004624=Homeobox=PD(64.7=75.0),PF0379811=TRAM_LAG1_CLN8=PU(2.6=11.4)
A:
NA
C2:
PF0379811=TRAM_LAG1_CLN8=FE(9.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCAGACTCCAGGCATTCAGA
R:
GCCACAGAAAACGCATCCCAT
Band lengths:
185-2151
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]